Strep鈥ag庐 Technology
Strep鈥ag庐 Technology Strep鈥ag庐 technology is based on the principle of the well-known strong
and specific interaction between biotin and streptavidin. To take advantage
Advantages of this interaction for recombinant protein affinity purification, we engineered
an 8-amino acid Strep鈥ag II fusion tag. To optimize Strep鈥ag II interaction
鈥? Bacterial, insect and mammalian expression
with the biotin-binding pocket of streptavidin, we developed Strep鈥actin庐
vectors, including convenient Ligation-
protein, a streptavidin derivative. The binding affinity of Strep鈥ag II to
independent Cloning (LIC) versions
Strep鈥actin is nearly 100 times higher than to streptavidin.
鈥? Rapid one-step affinity purification from
crude lysates under physiological conditions
The Strep鈥actin family of products offers a wide variety of supports for
鈥? Very high yields, greater than 95% fusion
rapid one-step affinity purification of proteins containing the Strep鈥ag II
protein purity and bioactivity
fusion tag. The Strep鈥ag II sequence binds to Strep鈥actin, and after unbound
鈥? Small 8 amino acids Strep鈥ag II does not
proteins are washed away, the purified target protein is competitively eluted
interfere with protein folding or activity
with 2.5 mM desthiobiotin, a reversibly binding analog of biotin. All purifica-
鈥? Resin is reusable 3-5 times
tion steps are performed under physiological conditions in PBS or other physi-
鈥? Purification procedure is compatible with
ological buffers.
a broad range of detergents, chelators and
salts
Expression Vectors with Strep鈥ag II
鈥? Avoids interactions with heavy metal ions
鈥? pET expression vectors with the powerful T7lac
which may catalyze protein oxidation
promoter for bacterial expression
鈥? Suitable for protein-protein interaction
鈥? InsectDirect鈩? expression vectors for rapid, virus-free
studies
high-level direct expression in insect cells
鈥? Over 50 literature citations using the
鈥? Multisystem expression vectors containing three promoters for
Strep鈥ag technology for expression &
high-level expression in bacterial, insect, and mammalian cells
purification of metalloproteins, membrane
鈥? All vectors are available in the convenient and efficient
proteins, and sensitive complexes with
Ligation-independent Cloning (LIC) format
multiple subunits
Strep鈥actin Purification and Detection Systems
鈥? Strep鈥actin Superflow鈩? and MacroPrep庐 resins for gravity flow
and low pressure chromatography
鈥? Prepackaged columns and cartridges for convenient handling
of multiple samples
鈥? 96-well cartridge plates for robotic systems
鈥? Strep鈥ag II Monoclonal Antibody, HRP conjugate and Strep鈥ag II
Perfect Protein鈩? Markers for recombinant protein detection
Strep鈥ag Technology for Protein-Protein Interaction Studies
鈥? Isolate intact protein complexes from mammalian cells in a single step
with One鈥TrEP鈩? Protein Interaction Kit
鈥? Achieve minimum background as compared to other tandem
Orders:
affinity purification (TAP) systems
Phone 800 854 3417
Fax 800 776 0999 鈥? Single-step procedure reduces sample dilution preventing the loss
of transient and weak protein interactions
Technical Support
210069
Phone 800 207 0144
New Strep鈥ag庐 System Products
Product Catalog No. Size Price
Strep鈥ag Puri铿乧ation Reagents & Kits*
Introductory Strep鈥ag庐 II Kit, pET-51b 71615-3 1 kit $ 285
Introductory Strep鈥ag II Kit, pET-52b 71616-3 1 kit $ 285
Strep鈥actin庐 Super铿俹w鈩? Agarose 71592-3 4 ml $ 132
Strep鈥actin Super铿俹w Agarose 71592-4 20 ml $ 518
Strep鈥actin Super铿俹w Column, 1 ml 71593-3 5 columns $ 553
Strep鈥actin Super铿俹w Column, 0.2 ml 71594-3 5 columns $ 300
Strep鈥actin Super铿俹w Cartridge, 1 ml 71595-3 5 cartridges $ 680
Strep鈥actin Super铿俹w Cartridge, 5 ml 71596-3 1 cartridge $ 370
Strep鈥actin Super铿俹w Cartridge, 5 ml 71596-4 5 cartridges $ 1,768
Strep鈥actin MacroPrep庐 Resin 71597-3 4 ml $ 132
Strep鈥actin MacroPrep Resin 71597-4 20 ml $ 518
Strep鈥actin MacroPrep Cartridge, 1 ml 71598-3 5 cartridges $ 670
Strep鈥actin HT96TM Puri铿乧ation Kit 71605-3 1 kit $ 465
Strep鈥actin庐 SpinPrepTM Kit 71608-3 25 rxn $ 269
M6 Adaptor Set for FPLC 71586-3 $ 102
1/4-28 Adaptor Set for FPLC 71587-3 $ 102
10-32 Adaptor Set for HPLC and 脛kta 71588-3 $ 88
1/16 Inch Adaptor Set for peristaltic pump tubing 71589-3 $ 76
Strep鈥actin Buffer Kit 71613-3 1 kit $ 111
10X Strep鈥actin Wash Buffer 71603-3 100 ml $ 51
10X Strep鈥actinElution Buffer 71604-3 25 ml $ 63
10X Strep鈥actin Regeneration Buffer 71612-3 100 ml $ 63
Biotin Blocking Buffer 71609-3 2 ml $ 88
D-Desthiobiotin 71610-3 1g $ 202
Strep鈥ag II Vector DNAs & LIC Vector Kits
Vectors for Bacterial Expression
pET-51b(+) DNA 71553-3 10 ug $ 147
pET-51 Ek/LIC Vector Kit 71570-3 1 kit $ 379
pET-52b(+) DNA 71554-3 10 ug $ 147
pET-52 3C/LIC Vector Kit 71571-3 1 kit $ 379
Vectors for Insect Expression
pIEx鈩?-8 DNA 71555-3 20 ug $ 219
pIEx-8 Ek/LIC Vector Kit 71572-3 1 kit $ 353
pIEx-9 DNA 71556-3 20 ug $ 219
pIEx-9 3C/LIC Vector Kit 71573-3 1 kit $ 353
pIEx-10 DNA 71557-3 20 ug $ 219
pIEx-10 Ek/LIC Vector Kit 71574-3 1 kit $ 353
Multisystem Expression Vectors
pTriEx鈩?-5 DNA 71558-3 20 ug $ 219
pTriEx-5 Ek/LIC Vector Kit 71575-3 1 kit $ 361
pTriEx-6 DNA 71559-3 20 ug $ 219
pTriEx-6 3C/LIC Vector Kit 71576-3 1 kit $ 361
pTriEx-7 DNA 71560-3 20 ug $ 219
pTriEx-7 Ek/LIC Vector Kit 71577-3 1 kit $ 361
Detection Reagents for Strep鈥ag II*
Strep鈥ag II Perfect Protein鈩? Markers, 16-100 kDa, Lyophilized 71614-3 100 lanes $ 152
Strep鈥ag II Monoclonal Antibody (suf铿乧ient for 50 blots) 71590-3 100 ug $ 260
Strep鈥ag II Antibody HRP Conjugate (suf铿乧ient for 25-30 blots) 71591-3 75 ul $ 210
Protein Interaction*
One鈥TrEP鈩? Protein Interaction Kit 71624-3 1 kit $990
One鈥TrEP鈩? is a trademark; and Strep鈥ag庐 and StrepTactin庐 are registered trademarks of IBA GmbH. Strep鈥ag products are provided for
research use only. Information about license for commercial use is available from IBA GmbH, Rudolf-Wissell-Str. 28, D-37079 Goettingen,
Germany.
* manufactured by IBA
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