SIGMA-ALDRICH
Material Safety Data Sheet
Date Printed: 07/JUN/2005
Date Updated: 13/MAR/2004
Version 1.3
According to 91/155/EEC
1 - Product and Company Information
Product Name FLUORESCEIN SODIUM
Product Number F6377
Company Sigma-Aldrich Pty. Ltd.
12 Anella Avenue
Castle Hill NSW 2154
Australia
Technical Phone # +61 2 9841 0555 (1800 800 097)
Fax +61 2 9841 0500 (1800 800 096)
Emergency Phone # +44 8701906777 (1800 448 465)
2 - Composition/Information on Ingredients
Product Name CAS # EC no Annex I
Index Number
FLUORESCEIN SODIUM 518-47-8 208-253-0 None
Formula C20H10Na2O5
Molecular Weight 376.28 AMU
Synonyms Aizen uranine * Calcocid uranine B4315 *
9-o-Carboxyphenyl-6-hydroxy-3-isoxanthone,
disodium salt * Certiqual fluoresceine * C.I.
766 * C.I. Acid Yellow 73 * C.I. 45350 disodium
salt * C.I. 45350 sodium salt * D&C Yellow No. 8
* Disodium 6-hydroxy-3-oxo-9-xanthene-o-benzoate
* Fluorescein sodium * Fluorescein sodium B.P *
Fluorescein, soluble * Fluor-I-strip A.T. *
Ful-Glo * Funduscein * Furanium * Hidacid
uranine * NCI-C54706 * Resorcinol phthalein
sodium * Sodium fluorescein * Sodium
fluoresceinate * Sodium salt of
hydroxy-o-carboxy-phenyl-fluorone * Soluble
fluorescein * Soluble fluoresceine *
Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one,
3',6'-dihydroxy-, disodium salt * Uranin *
Uranine * Uranine A Extra * Uranine O * Uranine
SS * Uranine USP XII * Uranine WSS * Uranine
Yellow * 11824 Yellow * 12417 Yellow
3 - Hazards Identification
SPECIAL INDICATION OF HAZARDS TO HUMANS AND THE ENVIRONMENT
Not hazardous according to Directive 67/548/EEC.
4 - First Aid Measures
AFTER INHALATION
If inhaled, remove to fresh air. If breathing becomes difficult,
call a physician.
AFTER SKIN CONTACT
In case of contact, immediately wash skin with soap and copious
amounts of water.
AFTER EYE CONTACT
In case of contact with eyes, flush with copious amounts of
water for at least 15 minutes. Assure adequate flushing by
separating the eyelids with fingers. Call a physician.
AFTER INGESTION
If swallowed, wash out mouth with water provided person is
conscious. Call a physician.
5 - Fire Fighting Measures
EXTINGUISHING MEDIA
Suitable: Water spray. Carbon dioxide, dry chemical powder, or
appropriate foam.
SPECIAL RISKS
Specific Hazard(s): Emits toxic fumes under fire conditions.
SPECIAL PROTECTIVE EQUIPMENT FOR FIREFIGHTERS
Wear self-contained breathing apparatus and protective clothing
to prevent contact with skin and eyes.
6 - Accidental Release Measures
PROCEDURE(S) OF PERSONAL PRECAUTION(S)
Exercise appropriate precautions to minimize direct contact with
skin or eyes and prevent inhalation of dust.
METHODS FOR CLEANING UP
Sweep up, place in a bag and hold for waste disposal. Avoid
raising dust. Ventilate area and wash spill site after material
pickup is complete.
7 - Handling and Storage
HANDLING
Directions for Safe Handling: Avoid inhalation. Avoid contact
with eyes, skin, and clothing. Avoid prolonged or repeated
exposure.
STORAGE
Conditions of Storage: Keep tightly closed.
SPECIAL REQUIREMENTS: Hygroscopic.
8 - Exposure Controls / Personal Protection
ENGINEERING CONTROLS
Safety shower and eye bath. Mechanical exhaust required.
GENERAL HYGIENE MEASURES
Wash thoroughly after handling.
PERSONAL PROTECTIVE EQUIPMENT
Respiratory Protection: Wear dust mask.
Hand Protection: Protective gloves.
Eye Protection: Chemical safety goggles.
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9 - Physical and Chemical Properties
Appearance Physical State: Solid
Property Value At Temperature or Pressure
pH N/A
BP/BP Range N/A
MP/MP Range N/A
Flash Point N/A
Flammability N/A
Autoignition Temp N/A
Oxidizing Properties N/A
Explosive Properties N/A
Explosion Limits N/A
Vapor Pressure N/A
SG/Density N/A
Partition Coefficient N/A
Viscosity N/A
Vapor Density N/A
Saturated Vapor Conc. N/A
Evaporation Rate N/A
Bulk Density N/A
Decomposition Temp. N/A
Solvent Content N/A
Water Content N/A
Surface Tension N/A
Conductivity N/A
Miscellaneous Data N/A
Solubility N/A
10 - Stability and Reactivity
STABILITY
Stable: Stable.
Conditions to Avoid: Moisture.
Materials to Avoid: Strong oxidizing agents.
HAZARDOUS DECOMPOSITION PRODUCTS
Hazardous Decomposition Products: Carbon monoxide, Carbon dioxide.
HAZARDOUS POLYMERIZATION
Hazardous Polymerization: Will not occur
11 - Toxicological Information
RTECS NUMBER: LM5425000
ACUTE TOXICITY
LD50
Oral
Rat
6721 mg/kg
Remarks: Behavioral:Change in motor activity (specific assay).
Behavioral:Ataxia. Lungs, Thorax, or Respiration:Dyspnea.
LD50
Intravenous
Rat
1 GM/KG
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Remarks: Blood:Other changes.
LD50
Oral
Mouse
4738 mg/kg
Remarks: Behavioral:Change in motor activity (specific assay).
Behavioral:Ataxia. Lungs, Thorax, or Respiration:Dyspnea.
LD50
Intravenous
Mouse
1 GM/KG
LD50
Intravenous
Dog
1 GM/KG
Remarks: Gastrointestinal:Changes in structure or function of
salivary glands.
SIGNS AND SYMPTOMS OF EXPOSURE
To the best of our knowledge, the chemical, physical, and
toxicological properties have not been thoroughly investigated.
ROUTE OF EXPOSURE
Skin Contact: May cause skin irritation.
Skin Absorption: May be harmful if absorbed through the skin.
Eye Contact: May cause eye irritation.
Inhalation: May be harmful if inhaled. Material may be
irritating to mucous membranes and upper respiratory tract.
Ingestion: May be harmful if swallowed.
TARGET ORGAN INFORMATION
Kidneys.
CHRONIC EXPOSURE - CARCINOGEN
Rat
Route of Application: Subcutaneous
Exposure Time: 79W
Result: Tumorigenic:Equivocal tumorigenic agent by RTECS
criteria. Tumorigenic:Tumors at site or application.
Rat
Route of Application: Subcutaneous
Exposure Time: 1Y
Result: Tumorigenic:Equivocal tumorigenic agent by RTECS
criteria. Skin and Appendages: Other: Tumors. Tumorigenic:Tumors
at site or application.
CHRONIC EXPOSURE - MUTAGEN
Mouse
200 MG/L
Cell Type: lymphocyte
Mutation in mammalian somatic cells.
Hamster
50100 UG/L
Cell Type: ovary
Sister chromatid exchange
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CHRONIC EXPOSURE - REPRODUCTIVE HAZARD
Species: Rabbit
Dose: 933 MG/KG
Route of Application: Intravenous
Exposure Time: (5-8D PREG)
Result: Maternal Effects: Postpartum. Effects on Newborn:
Viability index (e.g., # alive at day 4 per # born alive).
12 - Ecological Information
ECOTOXICOLOGICAL EFFECTS
Test Type: EC50 Daphnia
Species: Daphnia pulex
Time: 48 h
Value: 337 mg/l
Test Type: LC50 Fish
Species: Lepomis macrochirus (Bluegill)
Time: 96 h
Value: 3,433 mg/l
Test Type: LC50 Fish
Species: Onchorhynchus mykiss (Rainbow trout)
Time: 96 h
Value: 1,372 mg/l
13 - Disposal Considerations
SUBSTANCE DISPOSAL
Contact a licensed professional waste disposal service to dispose
of this material. Dissolve or mix the material with a combustible
solvent and burn in a chemical incinerator equipped with an
afterburner and scrubber. Observe all federal, state, and local
environmental regulations.
14 - Transport Information
RID/ADR
Non-hazardous for road transport.
IMDG
Non-hazardous for sea transport.
IATA
Non-hazardous for air transport.
15 - Regulatory Information
CLASSIFICATION AND LABELING ACCORDING TO EU DIRECTIVES
S-PHRASES: 22 24/25
Do not breathe dust. Avoid contact with skin and eyes.
Not hazardous according to Directive 67/548/EEC.
COUNTRY SPECIFIC INFORMATION
Germany
WGK: 2
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16 - Other Information
WARRANTY
The above information is believed to be correct but does not
purport to be all inclusive and shall be used only as a guide. The
information in this document is based on the present state of our
knowledge and is applicable to the product with regard to
appropriate safety precautions. It does not represent any
guarantee of the properties of the product. Sigma-Aldrich Inc.,
shall not be held liable for any damage resulting from handling or
from contact with the above product. See reverse side of invoice
or packing slip for additional terms and conditions of sale.
Copyright 2005 Sigma-Aldrich Co. License granted to make unlimited
paper copies for internal use only.
DISCLAIMER
For R&D use only. Not for drug, household or other uses.
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