MATERIAL SAFETY DATA SHEET
IDENTIFICATION
Stratagene Cloning Systems Date of last update: 6/16/00
11011 N. Torrey Pines Rd. Phone #: 800-894-1304
La Jolla, CA 92037 Fax #: 858-535-0071
Catalog # : 1012-13 Chloroform
CAS#: 67-66-3
HAZARDOUS COMPONENTS
Chemical Name & Synonyms: Chloroform (Chloroforme; Cloroformio; Formyl Trichloride; Mehtane
trichloride; Methane, trichloro; Methenyl trichloride; methyl trichloride; NCI-C02686; R 20; RCRA Waste
Number U044; TCM; Trichloromethaan; Trichlormethan; Trichloroform; Trichloromethane;
Triclorometano; UN1888 [DOT])
OSHA PEL Limits: 8 Hr. TWA: 1000 ppm (1900 mg/m3)
ACGIH TLV: TWA: 1000 ppm (1880 mg/m3)
OTHER LIMITS RECOMMENDED: N/A
KIT Chloroform -containing component %
餂?
720200-63 Chloroform 100
720200 Big Blue DNA Isolation Kit
TOXICITY DATA
Irritation Data:
SKN-RBT 10 mg/24Hr. Open MLD AIHAAP 23,95,62
SKN-RBT 500 mg/24Hr. MLD 85JCAE -, 89,86
EYE-RBT 148 mg AIHAAP 37,697,76
EYE-RBT 20 mg/ 85JCAE -, 89,86
Toxicity Data:
ORL-MAN LDLo: 2514 mg/kg AJEMEN 6,507,88
IHL-HMN LDLo: 25000 ppm/5m TABIA2 3,231,33
UNR-MAN LDLo: 546 mg/kg 85DCAI 2,73,70
ORL-RAT LD50:908 mg/kg JPFCD2 17,205,82
IHL-RAT LC50: 47720 mg/m3/4 hrs ENVRAL 40,411,86
IPR-RAT LD50: 849 mg/kg ENVRAL 40,411,86
ORL-MUS LD50: 36 mg/kg ATSUDG 2,371,79
IPR-MUS LD50: 623 mg/kg AGGHAR 18,109,60
SCU-MUS LD50: 704 mg/kg JPETAB 123,224,58
IPR-DOG LD50: 1 g/kg TXAPA9 10,119,67
SKN-RBT LD50: >20 g/kg NTIS** AD-A062-138
ORL-GPG LD50: 820 mg/kg GISAAA 48(3), 10,83
Target Organ Data:
Behavioral (Food Intake)
Liver (Liver function tests as impaired)
Blood (Other changes)
Effects on fertility (Female fertility index)
Effects on fertility (Post-implantation mortality)
Effects on fertility (Other measures of fertility)
Effects on embryo or fetus (Fetotoxicity)
Effects on embryo or fetus (Fetal death)
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Specific Developmental Abnormalities (Craniofacial)
Specific Developmental Abnormalities (Musculoskeletal system)
Specific Developmental Abnormalities (Gastrointestinal system)
Specific Developmental Abnormalities (Homeostasis)
Nutritional and Gross Metabolic (Weight loss or decreased weight gain)
Only selected registry of toxic effects of chemical substances (RTECS) data is presented here.
See actual entry in RTECS for complete information.
Reviews, Standards, and Regulations:
ACGIH TLV-Suspected human carcinogen. 85INA8 6,289,91
ACGIH TLV-TWA 10 ppm 85INA8 6,289,91
IARC CANCER REVIEW: Animal Sufficient evidence IMEMDT 20,401,79
IARC CANCER REVIEW: Human Inadequate evidence IMEMDT 20,401,79
IARC CANCER REVIEW: Group 2B IMSUDL 7,152,87
MSHA Standard: Air-CL 50 ppm (240 mg/m3) DTLWS* 3,14,73
OSHA PEL (Gen. Indu.): CL 50 ppm (240 mg/m3) CFRGBR 29,1910.1000,93
OSHA PEL (Fed. Cont.): CL 50 ppm (240 mg/m3) CFRGBR 41,50-204.50,94
NIOSH Rel. to Chloroform-Air: CA STEL 2 ppm/60 m3 DHHS #92-100,92
NIOSH Rel. to Waste Anesthetic Gases and Vapors-
Air: CL 2 ppm/hr MMWR** 37(S07),28,88
EPA Genetox program 1988, Positive: Carcinogenicity-Mouse/Rat; S. Cervisiae gene
conversion .
EPA Gentox Program 1988, Negative: cell transform. -SA7/SHE; v79 cell culture-gene mutation.
EPA TSCA Chemical Inventory, June 1993
EPA TSCA SECTION 8(E) Status report 8EHQ-0979-0310; 8EHQ-0180-0324 on EPA IRIS
database.
EPA TSCA Test Submission (TSCATS) data base, July 1994.
NIOSH current intelligence bulletin 9, 1976
NIOSH Analytical method, 1994: Hydrocarbons, Halogenated, 1003
NCI Carcinogenesis Bioassay (GAVAGE); Clear Evidence: mouse, rat NCITR* NCI-TR, 1976
NTP 7th Annual Report on Carcinogens, 1992: Anticipated to be carcinogen.
OSHA Analytical method #05
This product is subject to SARA Section 313 reporting requirements.
This product is, or contains chemical(s) known to the State of California to cause cancer.
Health Hazard Data
Acute Effects:
May be fatal if inhaled, swallowed or absorbed through skin.
Vapor or mist is irritating to the eyes, mucous membranes and upper respiratory tract.
Causes skin irritation.
Exposure can cause:
Nausea, dizziness and headache.
May cause nervous system disturbances.
Chronic Effects:
Carcinogen.
May alter genetic material.
Exposure to and/or consumption of alcohol may increase toxic effects.
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Target Organs:
Nerves
Heart
Liver
Kidneys
To the best of our knowledge, the chemical, physical and toxicological properties have not been
thoroughly investigated.
First Aid:
Eye contact: Immediately flush with copious amounts of water for at least 15 minutes.
Skin contact: Immediately flush with copious amounts of water for at least 15 minutes. Remove
contaminated clothing and shoes.
Inhalation: Remove to fresh air. If not breathing, give artificial respiration. If breathing is
difficult, give oxygen.
Ingestion: Provided person is conscious, wash out mouth with water. Call a physician
immediately!!!
Physical Data
Appearance and Odor:
Clear colorless liquid with a strong, sweet odor
Boiling point: 60.5掳C to 61.5掳C
Melting point: -63掳C
Flash point: None
Vapor Pressure: 160mmHg @ 20掳C
Specific gravity: 1.492 (H2O=1)
Fire and Explosion Hazard Data
Extinguishing Media:
Noncombustible. Use extinguishing media appropriate to the surrounding fire conditions.
Special Firefighting Procedures:
Wear self-contained breathing apparatus and protective clothing to prevent contact with skin and
eyes.
Unusual Fire and Explosion Hazards:
Emits toxic fumes under fire conditions.
Reactivity Data
Incompatibilities:
Bases
Aluminum
Sensitive to light
Hazardous Combustion or Decomposition Products:
Toxic fumes of:
Hydrogen Chloride Gas
Phosgene Gas
Carbon Monoxide, Carbon Dioxide
Stability:
Stable
Hazardous polymerization:
Will not occur.
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Spill or Leak Procedures
Steps to be taken if Material is Released or Spilled:
Evacuate area.
Wear self-contained breathing apparatus, rubber boots and heavy rubber gloves.
Absorb on sand or vermiculite and place in sealed containers until disposal.
Ventilate area and wash spill site after material pick up is complete.
Waste Disposal Method:
The material should be ignited in the presence of sodium carbonate and slaked lime (calcium
hydroxide). The substance should be mixed with vermiculite and then with the dry caustics,
wrapped in paper and burned in a chemical incinerator equipped with an afterburner and scrubber.
Observe all federal, state and local environmental regulations.
Precautions to be Taken in Handling and Storage
Label Precautionary Statements:
Highly toxic (USA)
Toxic (EC)
May cause cancer.
May cause heritable genetic damage.
Toxic by inhalation, in contact with skin and if swallowed.
Irritating to eyes, respiratory system and skin.
In case of accident, seek medical help immediately show label where possible.
Wear suitable protective clothing, gloves and eye/face protection.
Do not breathe vapor.
The above information is believed to be correct but does not purport to be all-inclusive and shall be used
only as a guide. Stratagene shall not be held liable for any damage resulting from handling or from contact
with the above product.
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