MATERIAL SAFETY DATA SHEET TBE BUFFER
PRODUCT CODE NUMBER(S): 8690-5
PRODUCT IDENTIFICATION reacts violently with acetic anhydride, potassium.
Incompatible with alkali carbonates, hydroxides.
Chemical Name and Synonyms: TBE Buffer, pH 8.0 Reactivity: Avoid excessive heat, generation of mist, all in-
Chemical Family: Mixture compatible materials.
Chemical Formula: Mixture Hazardous Decomposition Products: COx , NOx, borates
Product Use: Laboratory reagent
FIRE AND EXPLOSION DATA
Manufacturer鈥檚 Name and Address:
Caledon Laboratories Ltd. Flammability: Not combustible
40 Armstrong Avenue Extinguishing Media: Use extinguishing media
Georgetown, Ontario L7G 4R9 pappropriate to the surrounding fire. Water spray can be
used to cool containers, flush chemical away from fire. Fight
Telephone No: (905) 877-0101
fire from upwind, from a safe distance. Firefighters must
Fax No: (905) 877-6666
wear protective equipment and clothing sufficient to prevent
Emergency Telephone No: CANUTEC (613) 996-6666 inhalation of mist or fumes, and contact with skin and eyes.
Flash Point (Method Used): Not available
HAZARDOUS INGREDIENTS OF MATERIALS
Autoignition Temperature: Not available
Upper Flammable Limit (% by volume): Not available
Ingredients % TLV Units CAS No.
Lower Flammable Limit (% by volume): Not available
3
Tris(hydroxymethyl) N/A 10 mg/m 77-86-1
Hazardous Combustion Products: COx, NOx, irritating
methylamine (nuisance dust)
smoke, borates
Boric acid N/A Not established 10043-35-3
Sensitivity to Impact: None identified
Ethylenediamine Sensitivity to Static discharge: None identified
tetraacetic acid N/A Not established 6381-92-6
TOXICOLOGICAL PROPERTIES AND HEALTH DATA
PHYSICAL DATA
Toxicological Data:
Physical State: Liquid
LD50: (oral, rat) 5,900 mg/kg (Tris); (oral, rat) 2,000 mg/kg
Odour and Appearance: Colourless liquid
(EDTA); (oral, rat) 2,500 mg/kg (boric acid)
Odour Threshold (ppm): Not available
LC50: (rat) 16 mg/L (boric acid)
Vapour Pressure (mm Hg): Similar to water
Vapour Density (Air = 1): Similar to water Effects of Acute Exposure to Product:
Evaporation Rate: Similar to water
Inhaled: Mist may be irritating to upper respiratoy tract.
Boiling Point (degrees C): Similar to water Large amounts may be harmful. Severe overexposure to
Melting Point (degrees C): Similar to water boric acid can be harmful, causing shortness of breath,
cyanosis, respiratory depression.
pH: ~ 8.0
In contact with skin: May be irritating to skin. Severe
Specific Gravity: Similar to water
overexposure may cause drying and cracking of skin. Ab-
Coefficient of Water/Oil distribution: Similar to water
sorption of boric acid into blood stream through damaged
skin may result in erythema, macular rash, central nervous
SHIPPING DESCRIPTION system effects after 24 hours.
In contact with eyes: May cause mild irritation with tem-
UN: Not regulated
porary redness, itching, tearing, pain.
T.D.G. Class: Not regulated
Ingested: Large oral dosages may produce gastrointestinal
Pkg. Group: Not regulated
disturbances, nausea, vomiting, confusion, muscular
spasms, eventual coma. Large doses of boric acid may
REACTIVITY DATA cause circulatory collapse, tachycardia, cyanosis, delirium,
convulsions and coma. Death has been reported to occur in
Chemical Stability: Stable under normal temperatures and
adults from doses of 5 to 20 grams.
pressures
Incompatibility with other substances: May react vigor-
Effects of Chronic Exposure to Product:
ously with bases, oxidizing agents, acids. Tris reacts with
aldehydes to form bicyclic oxazolidines. Attacks copper, Carcinogenicity: No information available
brass, aluminum, but not steel or iron. Aluminum reacts with
Teratogenicity: Boric acid is a possible teratogen (RTECS
aqueous solutions of EDTA to release hydrogen. Boric acid
No. ED4550000)
TBE BUFFER
MSDS
CODE: 8690-5
Reproductive Effects: Tests with boric acid on laboratory ger down the throat. When vomiting occurs, rinse mouth
animals indicate that overexposure may cause reproductive and give more water to drink. Get medical attention.
disorders. Note to physician: Gastric lavage with 5% sodium bicar-
Mutagenicity: No information available bonate, followed by saline catharsis, is suggested after
ingestion of boric acid. Assure adequate hydration. After
Synergistic Products: Not known
ingestion of 10 g or more, symptoms may occur after 24 to
72 nours.
PREVENTIVE MEASURES
REFERENCES USED
Engineering Controls: Local exhaust ventilation recom-
mended.
CCINFO disc: Cheminfo
Respiratory Protection: Dust/mist mask. For conditions
Budavari: The Merck Index, 12th ed., 1997
where dust or mist is present, to the maximum use specified
by the respirator supplier, NIOSH/OSHA approved half- face Royal Society of Chemistry: Chemical Safety Data Sheets,
high-efficiency dust/mist filter respirator, or NIOSH/OSHA Vol. 2, 1989
approved full face-piece high-efficiency dust/mist filter respi- Sax, Lewis: Hawley鈥檚 Condensed Chemical Dictionary, 11th
rator. Higher or unknown concentrations, or for fire or spill
ed., 1987
conditions, self-contained breathing apparatus, or full
Suppliers鈥? Material Safety Data Sheets
face-piece, positive-pressure supplied-air respirator.
Eye Protection: Chemical safety glasses. Do not wear
ADDITIONAL INFORMATION
contact lenses when working with chemicals.
Skin Protection: Rubber gloves. Lab coat or apron with Date Issued: May 6, 1999
sleeves.
Revision: May 2008
Other Personal Protective Equipment: Eyewash fountain
MSDS: 8690-5
ans safety shower in work area.
Proposed WHMIS Designation: D2A; D2B (boric acid)
Leak and Spill Procedure: Ventilate area. Cleanup per-
sonnel should be thoroughly trained in the hazards of this
material and should wear protective equipment and clothing Prepared by: Caledon Laboratories Ltd. (905) 877-0101
sufficient to prevent inhalation and contact with skin and Caledon Laboratories Ltd. believes the information contained
eyes. Prevent from entering sewers or waterways. Mix with herein is reliable and accurate. Caledon makes no warranty
sand or inert absorbent, collect and transfer carefully into la-
with respect thereto and expressly disclaims all liability for reli-
belled, covered container(s) for removal by disposal
ance thereon. Such information is solely for your consider-
company. Wash site of spillage thoroughly with water and
ation, investigation, and verification.
detergent.
Waste Disposal: Follow all federal, provincial and local
regulations for disposal.
Handling Procedures and Equipment: Workers using this
chemical must be properly trained in its hazards and its safe
use, and must wear appropriate protective equipment and
clothing. Follow routine safe handling procedures and good
housekeeping procedures. Avoid generating mist. Avoid in-
halation and contact with skin and eyes. Use the smallest
amount possible for the purpose, in designated areas with
adequate ventilation. Keep containers closed when not in
use and when empty. Wash thoroughly after handling.
Storage Requirements: Store in suitable, labelled contain-
ers, in a cool, dry, well-ventilated area, out of direct sunlight.
Store away from incompatible materials. Keep containers
tightly closed when not in use and when empty. Protect
from damage, and inspect frequently for signs of leaking.
FIRST AID MEASURES
Specific Measures:
Eyes: Flush eyes with warm running water for fifteen (15)
minutes, holding eyelids open during flushing. If irritation
persists, obtain medical advice.
Skin: Remove contaminated clothing. Wash skin with
plenty of water for at least ten to fifteen (10 to 15) minutes.
If there are any breaks in the affected skin, or if irritation de-
velops, get medical attention.
Inhalation: Remove to fresh air. Give oxygen and get
medical advice for any breathing difficulty.
Ingestion: If casualty is alert and not convulsing, give 2 to
4 glasses of water to dilute material. Induce vomiting under
medical supervision, with syrup of ipecac or by sticking a fin-
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