MATERIAL SAFETY DATA SHEET
For IPeX Immunoprecipitaion of Antigen Free from Antibody
1. PRODUCTS AND COMPANY IDENTIFICATION
Product Name: IPeX kit
Catalog Numbers: IP-10, IP-2 and IPR-100
Company Name & Address: Gene Bio-Application Ltd.
P. O.Box 206
Kfar Hanagid
Israel
Tel.: +972-8-9322623
Fax: +972-8-9322380
Information Number: Please contact Gene Bio-Application Ltd. distributor in your country.
2. COMPOSITION / INFORMATION ON INGREDIENTS
Description
Water solutions of components listed.
Volume of each product package varies: 350-2 ml.
Components in Water solution Concentration, M or % Present in kit buffer
1. Sodium Bicarbonate 0.3-0.6 M (A)
2. Tris-Base 1.2-1.7 M (C)
3. Dulbecco鈥檚 Phosphate saline 10X (D)
4. Potassium Chloride 0.8-1.2 M (E)
5. Ethylenediamine-Tetraacetic acid (EDTA) 2.0-8.0 mM (E)
6. Tris-Hcl 30-60 mM (E)
7. Triton X-100 0.2-0.8 % (E)
8. Glycine 1.3-1.6 M (F)
9. Sodium Chloride 1.8-2.2 M (E and GEB)
Solid Component
1. Sodium Borohydride (B)
List of Products Information
Product Name Formula Molecular Weight Cas # EC no
Sodium Bicarbonate NaHCO3 84.01 144-55-8 205-633-8
Trizma Base C4H11NO3 121.14 77-86-1 201-064-4
Dulbecco鈥檚 Phosphate Buffer
None None
Saline
Potassium Chloride KCl 74.56 7447-40-7 231-211-8
Ethylenediaminetetraactic Acid
C10H14N2Na2O8 ^ 2H2O 372.24 63831-92-6 205-358-3
Disodumedihydrate
Trizma Hydrochloride C4H11NO3 157.6 1185-53-1 214-684-5
Triton X-100 (C2H4O) nC14H22O 9002-93-1 None
Glycine Free Base C2H5NO2 75.07 56-40-6 200-272-2
Sodium Chloride NaCl 58.44 7647-14-5 231-598-3
Sodium Borohydride NaBH4 37.83 16940-66-2 241-004-4
3. HAZARDS IDENTIFICATION
Sodium Bicarbonate, Dulbecco鈥檚 Phosphate saline, Potassium Chloride, Glycine and Sodium
Chloride - Not hazardous according to Directive 67/548/EC.
Trizma Base, Ethylenediamine-Tetraacetic acid (EDTA) and Tris-Hcl -Irritating to eyes, and skin.
Triton X-100- Harmful if swallowed. Risk of serious damage to eyes.
Sodium Borohydride- Contact with water liberates extremely flammable gases. Toxic in contact with skin
and if swallowed. Very toxic by inhalation.
Gene Bio-Application Ltd. 01/09/04
4. FIRST AID MEASURES
After skin contact
Sodium Bicarbonate, Trizma Base, Dulbecco鈥檚 Phosphate saline, Potassium Chloride,
Ethylenediamine-Tetraacetic acid (EDTA), Tris-Hcl, Triton X-100, Glycine and Sodium Chloride - In
case of contact, immediately wash skin with soap and copious amount of water.
After Inhalation
Sodium Bicarbonate, Dulbecco鈥檚 Phosphate saline, Potassium Chloride, Triton X-100, Glycine and
Sodium Chloride 鈥? If inhaled, remove to fresh air. If breathing becomes difficult, call a physician.
Trizma Base, Ethylenediamine-Tetraacetic acid (EDTA), Tris-Hcl and Sodium Borohydride - If inhaled,
remove to fresh air. If not breathing, give artificial respiration. If breathing becomes difficult, give oxygen.
After eye contact
Sodium Bicarbonate, Trizma-Base, Dulbecco鈥檚 Phosphate saline, Potassium Chloride,
Ethylenediamine-Tetraacetic acid (EDTA), Tris-Hcl, Triton X-100, Glycine and Sodium Chloride 鈥? In
case of contact with eyes, flush with copious amounts of water for at least 15 minutes. Assure adequate
flushing by separating the eyelids with fingers. Call a physician.
After Ingestion
Sodium Bicarbonate, Trizma-Base, Dulbecco鈥檚 Phosphate saline, Potassium Chloride,
Ethylenediamine-Tetraacetic acid (EDTA), Tris-Hcl, Triton X-100, Glycine and Sodium Chloride - If
swallowed, wash out mouth with water provided person is conscious. Call a physician.
5. FIRE FIGHTING MEASURES
Extinguishing Media
Sodium Bicarbonate, Trizma-Base, Dulbecco鈥檚 Phosphate saline, Potassium Chloride,
Ethylenediamine-Tetraacetic acid (EDTA), Tris-Hcl, Glycine and Sodium Chloride - Suitable: Water
spray. Carbon dioxide, dry chemical powder or appropriate foam.
Triton X-100- Suitable: Water spray. Carbon dioxide, dry chemical powder or appropriate foam. Foam and
water spray are effective but may cause frothing.
Sodium Borohydride- Reacts with water to liberate flammable and/or explosive gas. Reacts with metals to
liberate flammable and/or hydrogen gas. Suitable: Dry chemical powder.
Unsuitable: Do not use water, foam, or carbon dioxide. Do not use
halocarbon extinguishers.
Special Risks
Sodium Bicarbonate, Trizma-Base, Dulbecco鈥檚 Phosphate saline, Potassium Chloride,
Ethylenediamine-Tetraacetic acid (EDTA), Tris-Hcl, Triton X-100, Glycine and Sodium Chloride 鈥?
Specific Hazard(s): Emits toxic fumes under fire conditions.
Sodium Borohydride- Emits toxic fumes under fire conditions. Explosion Hazards: Material readily reacts
with water generating flammable and/or explosive hydrogen gas.
Special Protective Equipment for Firefighting
Sodium Bicarbonate, Trizma-Base, Dulbecco鈥檚 Phosphate saline, Potassium Chloride,
Ethylenediamine-Tetraacetic acid (EDTA), Tris-Hcl, Triton X-100, Glycine, Sodium Chloride and
Sodium Borohydride 鈥? Wear self-contained breathing apparatus and protective clothing to prevent contact
with skin and eyes.
Gene Bio-Application Ltd. 01/09/04
6. ACCIDENTAL RELEASE MEASURES
Procedure of Personal Precautions
Sodium Bicarbonate, Dulbecco鈥檚 Phosphate saline, Potassium Chloride, Glycine and Sodium
Chloride鈥? Exercise appropriate precautions to minimize direct contact with skin or eyes.
Trizma-Base, Ethylenediamine-Tetraacetic acid (EDTA), Tris-Hcl and Triton X-100 and Sodium
Borohydride - Wear chemical safety goggles, rubber boots and heavy rubber gloves.
Personal Precaution Procedures to be Followed in Case of Leak or Spill
Trizma-Base- Sweep together the wetted material carefully and fill into marked, sealed containers.
Triton X-100 and Sodium Borohydride 鈥? Evacuate area.
Environmental Precautions
Potassium Chloride and Triton X-100 鈥? Avoid contaminating sewers and waterways with this material.
Methods for cleaning up
Sodium Bicarbonate, Trizma-Base, Dulbecco鈥檚 Phosphate saline, Potassium Chloride,
Ethylenediamine-Tetraacetic acid (EDTA), Tris-Hcl, Triton X-100, Glycine, Sodium Chloride and
Sodium Borohydride- Dry and absorb spill site and place in a bag and hold for waste disposal. Ventilate
area and wash spill site after material pickup is complete
7. HANDLING AND STORAGE
Handling
Sodium Bicarbonate, Trizma-Base, Dulbecco鈥檚 Phosphate saline, Potassium Chloride,
Ethylenediamine-Tetraacetic acid (EDTA), Tris-Hcl, Triton X-100, Glycine, Sodium Chloride and
Sodium Borohydride - Direction for safe handling: Avoid contact with eyes, skin and clothing. Avoid
prolonged or repeated exposure.
Storage
Sodium Bicarbonate, Trizma-Base, Dulbecco鈥檚 Phosphate saline, Potassium Chloride,
Ethylenediamine-Tetraacetic acid (EDTA), Tris-Hcl, Triton X-100, Glycine and Sodium Chloride - Keep
tightly close.
Sodium Borohydride- Keep tightly close. Store in a cool dry place.
Special Requirements
Sodium Borohydride- Reacts violently with water. Heat sensitive.
8. EXPOSURE CONTROLS / PERSONAL PROTECTION
Engineering Controls
Sodium Bicarbonate, Trizma-Base, Dulbecco鈥檚 Phosphate saline, Ethylenediamine-Tetraacetic acid
(EDTA), Tris-Hcl, Triton X-100, Glycine and Sodium Chloride鈥? Safety shower and eye bath. Mechanical
exhaust required.
Sodium Borohydride- Safety shower and eye bath. Use only in chemical fume hood.
General Hygiene Measures
Sodium Bicarbonate, Trizma-Base, Dulbecco鈥檚 Phosphate saline, Ethylenediamine-Tetraacetic acid
(EDTA), Tris-Hcl, Triton X-100, Glycine and Sodium Chloride鈥? Wash thoroughly after handling.
Gene Bio-Application Ltd. 01/09/04
Potassium Chloride and Sodium Borohydride - Wash thoroughly after handling. Wash contaminated
clothing before reuse.
Personal Protective Equipment
Sodium Bicarbonate, Trizma-Base, Dulbecco鈥檚 Phosphate saline, Potassium Chloride,
Ethylenediamine-Tetraacetic acid (EDTA), Tris-Hcl, Triton X-100, Glycine and Sodium Chloride 鈥?
Hand Protection: Chemical protective gloves.
Eye Protection: Chemical safety goggles.
Sodium Borohydride- Respiratory protection: Government approved respirator.
Hand Protection: Chemical protective gloves.
Eye Protection: Chemical safety goggles.
Skin Protection: Chemical resistant apron.
9. PHYSICAL AND CHEMICAL PROPERTIES
Appearance and Physical State
Sodium Bicarbonate, Trizma-Base, Potassium Chloride, Ethylenediamine-Tetraacetic acid (EDTA),
Tris-Hcl, Triton X-100, Glycine and Sodium Chloride - Water solution colorless.
Dulbecco鈥檚 Phosphate saline- N/A
Sodium Borohydride 鈥? Solid white color.
Extra Detailed Information
Sodium Bicarbonate- MP/MP Range: 300OC; SG/Density: 2.16 g/cm3.
Trizma-Base- BP/BP Range: 219-220OC at 10 mmHg; MP/MP Range: 171.2-172.30OC; Bulk Density 0.8
kg/l.
Potassium Chloride- BP/BP Range: 1500OC; MP/MP Range: 770OC; SG/Density: 1.98 g/cm3.
Ethylenediamine-Tetraacetic acid (EDTA) - MP/MP Range: 248OC.
Tris-Hcl- MP/MP Range: 150-152OC.
Triton X-100- BP/BP Range: > 200OC; Flash point: 251OC; Vapor pressure: < 1 mmHg at 20OC; SG/Density:
1.07 g/cm3; Vapor Density: > 1 g/l; Evaporation Rate: < 0.01.
Glycine- MP/MP Range: 240OC.
Sodium Chloride- BP/BP Range: 1413OC; MP/MP Range: 801OC; Vapor pressure: 1 mmHg at 865OC;
SG/Density: 2.165 g/cm3.
Sodium Borohydride - MP/MP Range: 400OC; Explosion Limits: lower: 3.02%; SG/Density: 1.07 g/cm3.
10. STABILITY AND REACTIVITY
Stability
Sodium Bicarbonate, Trizma-Base, Dulbecco鈥檚 Phosphate saline, Potassium Chloride,
Ethylenediamine-Tetraacetic acid (EDTA), Tris-Hcl, Triton X-100, Glycine, Sodium Chloride and
Sodium Borohydride - Stable, under normal handling and storage conditions.
Hazardous Decomposition Products
Sodium Bicarbonate, Dulbecco鈥檚 Phosphate saline and Triton X-100 - Carbon monoxide and Carbon
dioxide.
Gene Bio-Application Ltd. 01/09/04
Trizma-Base and Ethylenediamine-Tetraacetic acid (EDTA), - Carbon monoxide, Carbon dioxide and
Nitrogen oxides.
Potassium Chloride- Hydrogen chloride gas, Potassium oxides.
Tris-Hcl- Thermal decomposition may produce Carbon monoxide, Carbon dioxide, Nitrogen oxides and
Hydrogen chloride gas.
Glycine- Carbon monoxide, Carbon dioxide, Nitrogen oxides and Ammonia.
Sodium Chloride- Sodium/sodium oxides and Hydrogen chloride gas.
Sodium Borohydride- Borane/boron oxides, sodium oxide, Hydrogen gas. Hazardous decomposition
products formed upon contact with water: material readily reacts with water generating flammable and/or
explosive hydrogen gas.
Hazardous Polymerization
Sodium Bicarbonate, Trizma-Base, Dulbecco鈥檚 Phosphate saline, Potassium Chloride,
Ethylenediamine-Tetraacetic acid (EDTA), Tris-Hcl, Triton X-100, Glycine, Sodium Chloride and
Sodium Borohydride- will not occur
11. TOXICOLOGICAL INFORMATION
Sodium Bicarbonate- RTECS Number: VZ0950000; Acute Toxicity: LD50 Oral Rat 4220mg/kg, Oral Mouse
3360 mg/kg; Irritation Data: Skin Human 30 mg 3D I, Remarks: Mild irritation effect; Eyes rabbit 100 mg
30S Remarks: Mild irritation effect. Signs and symptoms of exposure: Exposure to large amounts can
cause: gastrointestinal disturbances. Heavy or prolonged skin exposure may result in the absorption of
harmful amounts of material. Route of Exposure: Skin contact: May cause skin irritation. Skin
Absorption: May be harmful if absorbed through the skin. Eye contact: May cause eye irritation.
Inhalation: May be harmful if inhaled. Material may be irritation to mucous membranes and upper respiratory
tract. Ingestion: May be harmful if swallowed. Conditions Aggravated by Exposure: Alkalosis, an
abnormal condition of increased alkalinity of the blood and tissues. Chronic Exposure 鈥? Mutagen: Rat
50400 mg/kg, Oral 4W, Unscheduled DNA synthesis. Chronic Exposure 鈥? Teratogen: Species: Mouse,
Dose: 40 mg/kg, Route of Application: Intraperitoneal, Exposure time: 7D PREG, Results: specific
Developmental Abnormalities: Other developmental abnormalities.
Trizma-Base- RTECS Number: TY2900000; Acute Toxicity: LD50 Oral Rat 5990mg/kg, Intravenous Rat
1800 mg/kg; LD50 Intravenous Mouse 1210 mg/kg. Signs and symptoms of exposure: To the best of our
knowledge, the chemical, physical, and toxicological properties have not been thoroughly investigated.
Route of Exposure: Skin contact: May cause skin irritation. Skin Absorption: May be harmful if absorbed
through the skin. Eye contact: May cause eye irritation. Inhalation: Material is irritation to mucous
membranes and upper respiratory tract. May be harmful if inhaled. Ingestion: May be harmful if swallowed.
Dulbecco鈥檚 Phosphate saline - Signs and symptoms of exposure: To the best of our knowledge, the
chemical, physical, and toxicological properties have not been thoroughly investigated. Route of Exposure:
Skin contact: May cause skin irritation. Skin Absorption: May be harmful if absorbed through the skin. Eye
contact: May cause eye irritation. Inhalation: Material is irritation to mucous membranes and upper
respiratory tract. May be harmful if inhaled. Ingestion: May be harmful if swallowed.
Potassium Chloride - RTECS Number: TS8050000; Acute Toxicity: LDLO Oral Infant 938mg/kg 2D.
Remarks:Lungs, Thorax, or Respiration: Cyanosis. Lungs, Thorax, or respiration: Other Changes. Nutritional
and Gross Metabolic: Changes in: K. LDLO Oral Man 20mg/kg. Remarks: cardiac: Arrythmias (including
changes it conduction). Gastrointestinal: Nausea or vomiting. Blood: Change in clotting factors. LD50 Oral
Rat 2600 mg/kg, LD50 Intraperitoneal Rat 660 mg/kg, LD50 Intravenous Rat 142 mg/kg. Remarks:
Behavioral: Convulsions or effect on seizure threshold. Lungs, Thorax, or Respiration: Dyspnea. LD50 Oral
Mouse 1500 mg/kg, LD50 Intraperitoneal Rat 620 mg/kg, LD50 Intravenous Rat 117 mg/kg. Irritation Data:
Eyes Rabbit 500 mg 24H, Remarks: Mild irritation effect. Signs and symptoms of exposure: Ingestion of
large quantities can cause weakness, gastrointestinal irritation, and circulatory disturbances. Route of
Gene Bio-Application Ltd. 01/09/04
Exposure: Skin contact: May cause skin irritation. Skin Absorption: May be harmful if absorbed through
the skin. Eye contact: May cause eye irritation. Inhalation: May be harmful if inhaled. Material may be
irritation to mucous membranes and upper respiratory tract. Ingestion: May be harmful if swallowed. Target
Organ- Heart. Chronic Exposure 鈥? Mutagen: Rat 1500 ug/kg, Oral Unscheduled DNA synthesis. Mouse
2048 mg/L (+S9), cell type: Lymphocyte, Mutation in microorganisms. Hamster 260 MMOL/l Cell type: ovary,
DNA damage. Hamster 12 gm/l Cell type: Lung, cytogenetic analysis. Hamster 140 MMOL/l Cell type: ovary,
cytogenetic analysis. Hamster 180 MMOL/l Cell type: ovary, Sister chromatid exchange.
Ethylenediamine-Tetraacetic acid (EDTA)- RTECS Number: AH4410000; Signs and symptoms of
exposure: To the best of our knowledge, the chemical, physical, and toxicological properties have not been
thoroughly investigated. Route of Exposure: Skin contact: May cause skin irritation. Skin Absorption:
May be harmful if absorbed through the skin. Eye contact: May cause eye irritation. Inhalation: Material is
irritation to mucous membranes and upper respiratory tract. May be harmful if inhaled. Ingestion: May be
harmful if swallowed. Chronic Exposure 鈥? Mutagen: Hamster 200 gm/l Cell type: Lung, cytogenetic
analysis.
Tris-Hcl- Signs and symptoms of exposure: To the best of our knowledge, the chemical, physical, and
toxicological properties have not been thoroughly investigated. Route of Exposure: Skin contact: May
cause skin irritation. Skin Absorption: May be harmful if absorbed through the skin. Eye contact: May
cause eye irritation. Inhalation: Material is irritation to mucous membranes and upper respiratory tract. May
be harmful if inhaled. Ingestion: May be harmful if swallowed.
Triton X-100- RTECS Number: MD0907700; Acute Toxicity: LD50 Oral Rat, female 707mg/kg; LD50 Oral
Rat, male 500mg/kg; LD50 Skin Rabbit, 12300mg/kg; LD50 Skin Rabbit 8000mg/kg; LD50 Oral Rat
1800mg/kg; LD50 Intravenous Mouse 1200mg/kg; Irritation Data: Eyes Rabbit 0.01 ml, Remarks: Severe
irritation effect. : Skin Rabbit 0.5 ml 24H, Remarks: Mild irritation effect. Eyes Rabbit 0.01 ml 24H, Remarks:
Moderate irritation effect. Signs and symptoms of exposure: To the best of our knowledge, the chemical,
physical, and toxicological properties have not been thoroughly investigated. Route of Exposure: Skin
contact: May cause skin irritation. Skin Absorption: May be harmful if absorbed through the skin. Eye
contact: May cause eye irritation. Inhalation: Material is irritation to mucous membranes and upper
respiratory tract. May be harmful if inhaled. Ingestion: May be harmful if swallowed. Chronic Exposure 鈥?
Mutagen: Human 21 mg/L, cell type: Hela cell, DNA inhibition; Human 14 mg/L, cell type: Hela cell, Other
mutation test system; Mouse 200 PPM, cell type: Ascites tumor, Unscheduled DNA synthesis; Chronic
Exposure 鈥? Reproductive Hazard: Species: Rat, Dose: 65500 mg/kg, Route of application: Oral, Exposure
Time: (26W PRE), Results: Maternal Effects: Ovaries, fallopian tubes. Species: Rat, Dose: 40 ml/kg, Route
of application: Skin, Exposure Time: (6-15D PRE), Results: Maternal Effects: Other effects. Specific
developmental abnormalities: Musculoskeletal system. Species: Rat, Dose: 5 mg/kg, Route of application:
Parenteral, Exposure Time: (1D PRE), Results: Maternal Effects: Ovaries, fallopian tubes.
Glycine- RTECS Number: MD0907700; Acute Toxicity: LD50 Oral Rat, 7930 mg/kg; LD50 Subcutaneous
Rat, 5200 mg/kg; LD50 Intravenous Rat, 2600 mg/kg; LD50 Oral Mouse, 4920 mg/kg; LD50 Intraperitoneal
Mouse, 4450 mg/kg; LD50 Subcutaneous Mouse, 5060 mg/kg; LD50 Intravenous Mouse, 2370 mg/kg;
Route of Exposure: Skin contact: May cause skin irritation. Skin Absorption: May be harmful if absorbed
through the skin. Eye contact: May cause eye irritation. Inhalation: Material is irritation to mucous
membranes and upper respiratory tract. May be harmful if inhaled. Ingestion: May be harmful if swallowed.
Chronic Exposure 鈥? Mutagen: Human 100 mg/L, cell type: Lymphocyte cell, Sister chromatid exchange.
Sodium Chloride- RTECS Number: VZ4725000; Acute Toxicity: LD50 Oral Rat, 3000 mg/kg; LD50
Inhalation Rat, > 42000 mg/m3 1H; LD50 Oral Mouse, 4000 mg/kg; LD50 Intraperitoneal Mouse, 2602mg/kg;
LD50 Subcutaneous Mouse, 3 gm/kg; LD50 Intravenous Mouse, 645 mg/kg; LD50 Intracervical Mouse, 131
mg/kg; LD50 Skin Rabbit, 10000mg/kg 24H; Irritation Data: Skin Rabbit 50 mg 24H, Remarks: Mild irritation
effect. Skin Rabbit 500 mg 24H, Remarks: Mild irritation effect. Eyes Rabbit 500 mg 24H, Remarks: Mild
irritation effect. Eyes Rabbit 100 mg 24H, Remarks: Mild irritation effect. Signs and symptoms of
exposure: Ingestion of large amounts causes vomiting and diarrhea. Dehydration and congestion may occur
in internal organs. Hypertonic salt solutions can produce inflammatory reactions in the gastrointestinal tract.
Route of Exposure: Skin contact: May cause skin irritation. Skin Absorption: May be harmful if absorbed
through the skin. Eye contact: NaCl cause eye irritation in contact also can cause irritation or redness due
to abrasion. Inhalation: Material is irritation to mucous membranes and upper respiratory tract. May be
harmful if inhaled. Ingestion: May be harmful if swallowed. Chronic Exposure 鈥? Mutagen: Human 125
Gene Bio-Application Ltd. 01/09/04
mmol/L, cell type: Fibroblast, DNA inhibition. Rat, Oral, 16800 mg/kg, Unscheduled DNA synthesis. Rat 400
mg/kg, Oral, Other mutation tests system. Rat 2338 mg/kg, Intraperitoneal, Cytogenetic analysis. Mouse 101
mmol/L, Cell type: Lymphocyte, DNA damage. Mouse 57200 umol/L, Cell type: Lymphocyte, Mutation in
mammalian somatic cells. Hamster 4 gm/L, Cell type: Lung, Micronucleus test. Hamster 275 mmol/L, Cell
type: Ovary, DNA damage. Hamster 160 mmol/L, Cell type: Ovary, Cytogenetic analysis. Hamster 7500
mg/L, Cell type: Lung, Cytogenetic analysis. Chronic Exposure 鈥? Teratogen: Species: Rat, Dose: 1710
mg/kg Route of application: Intraperitoneal, Exposure Time: (13D PREG) Results: Effects on Embryo or
Fetus: Fetotoxicity (except death, e.g., stunted fetus). Effects on Embryo or Fetus: Fetal death. Specific
developmental abnormalities: Musculoskeletal system. Species: Mouse, Dose: 1900 mg/kg Route of
application: Subcutaneous, Exposure Time: (11D PREG) Results: Effects on Embryo or Fetus: Fetal death.
Species: Mouse, Dose: 1900 mg/kg Route of application: Subcutaneous, Exposure Time: (10D PREG)
Results: Specific developmental abnormalities: Musculoskeletal system. Species: Mouse, Dose: 2500 mg/kg
Route of application: Subcutaneous, Exposure Time: (10D PREG) Results: Effects on embryo or fetus:
Fetotoxicity. Chronic Exposure 鈥? Reproductive Hazard: Species: Woman, Dose: 27 mg/kg Route of
application: Intraplacental, Exposure Time: (15W PREG) Results: Effects on Fertility: Abortion. Species: Rat,
Dose: 145 mg/kg Route of application: Oral, Exposure Time: (7D PRE/1-22D PREG) Results: Effects on
newborn: Delayed effect. Species: Rat, Dose: 56400 mg/kg Route of application: Oral, Exposure Time: (5D
PRE-21D POST) Results: Maternal effect: Postpartum. Effects on newborn: Biochemical and metabolic.
Species: Rat, Dose10 g/kg Route of application: Intraperitoneal, Exposure Time: (17-20D PREG) Results:
Effect on newborn: Behavioral. Species: Rat, Dose10 g/kg Route of application: Parenteral, Exposure Time:
(1D PRE) Results: Maternal effect: Ovaries, fallopian tubes. Species: Rat, Dose: 500 mg/kg Route of
application: Intrauterine, Exposure Time: (4D PREG) Results: Effect on fertility: Pre-implantation mortality
(e.g., reduction in number of implants per female; total number of implants per corpora lutea). Species: Rat,
Dose: 50 mg/kg Route of application: Intrauterine, Exposure Time: (6D PREG) Results: Effect on fertility:
Post-implantation mortality (e.g., dead and/or restored implants per total number of implants). Species:
Mouse, Dose: 13440 mg/kg Route of application: Subcutaneous, Exposure Time: (2-6D PREG) Results:
Effect on fertility: Abortion. Species: Monkey, Dose: 6 g/kg Route of application: Intrauterine, Exposure Time:
(18W PREG) Results: Effect on fertility: Abortion. Species: Horse, donkey, Dose: 480 mg/kg Route of
application: Intraplacental, Exposure Time: (45D PREG) Results: Maternal effect: Other effect: Endocrine:
Estrogenic. Effect on embryo or fetus: Fetal death.
Sodium Borohydride- RTECS Number: ED3325000; Acute Toxicity: LD50 Oral Rat, 162 mg/kg; Remarks:
sense organs and special Senses (Nose, Eye, Ear and Taste): Eye: Other. Behavioral: Convulsions or effect
on seizure threshold. Lungs, Thorax, or Respiration: Structural or functional change in trachea or bronchi.
LC50 Inhalation Rat, 36 mg/m3; Remarks: sense organs and special Senses (Nose, Eye, Ear and Taste):
Eye: Other. Behavioral: Convulsions or effect on seizure threshold. Lungs, Thorax, or Respiration: Structural
or functional change in trachea or bronchi. LD50, Intraperitoneal, Rat 18 mg/kg, LD50, Subcutaneous, Rat
177 mg/kg, Remarks: sense organs and special Senses (Nose, Eye, Ear and Taste): Eye: Other. Behavioral:
Convulsions or effect on seizure threshold. Lungs, Thorax, or Respiration: Structural or functional change in
trachea or bronchi. LD50, Oral, Mouse 50 mg/kg, Remarks: sense organs and special Senses (Nose, Eye,
Ear and Taste): Eye: Other. Behavioral: Convulsions or effect on seizure threshold. Lungs, Thorax, or
Respiration: Structural or functional change in trachea or bronchi. LD50, Oral, Rabbit 50 mg/kg, Remarks:
sense organs and special Senses (Nose, Eye, Ear and Taste): Eye: Other. Behavioral: Convulsions or effect
on seizure threshold. Lungs, Thorax, or Respiration: Structural or functional change in trachea or bronchi.
LD50, Skin, Rabbit 230 mg/kg, Remarks: sense organs and special Senses (Nose, Eye, Ear and Taste):
Eye: Other. Behavioral: Convulsions or effect on seizure threshold. Lungs, Thorax, or Respiration: Structural
or functional change in trachea or bronchi. Signs and symptoms of exposure: Material is extremely
destructive to tissue of the mucous membranes and upper respiratory tract, eyes, and skin. Inhalation may
result in spasm, inflammation and edema of the larynxand bronchi, chemical pneumonitis, and pulmonary
edema. Symptoms of exposure may include burning sensation, coughing, wheezing, laryngitis, shortness of
breath, headache, nausea, and vomiting. To the best of our knowledge, the chemical, physical, and
toxicological properties have not been thoroughly investigated. Route of Exposure: Skin contact: Causes
burns. Skin Absorption: Toxic if absorbed through skin. Eye contact: Causes burns. Inhalation: May be
fetal if inhaled. Material is extremely destructive to tissue of the mucous membranes and upper respiratory
tract. Ingestion: Toxic if swallowed.
12. ECOLOGICAL INFORMATION
Gene Bio-Application Ltd. 01/09/04
Sodium Bicarbonate, Trizma-Base, Dulbecco鈥檚 Phosphate saline, Potassium Chloride,
Ethylenediamine-Tetraacetic acid (EDTA), Tris-Hcl, Glycine, Sodium Chloride and Sodium
Borohydride - No data available.
Triton X-100- Elimination: 90%. Ecotoxicological Effects: Test type: IC50 Algae. Species: Other
microorganisms. Value: 5000 mg/l. Test type: EC50 Daphnia. Species: Daphnia. Time: 48 h, Value: 26 mg/l.
Test type: LC50 Fish. Species: Pimephales promelas. Time: 96 h, Value: 8.9 mg/l. Test type: LC50 Fish.
Species: Pimephales promelas. Time: 96 h, Value: 4.3-6 mg/l.
13. DISPOSAL CONSIDERATIONS
Substance Disposal- Sodium Bicarbonate, Trizma-Base, Dulbecco鈥檚 Phosphate saline, Potassium
Chloride, Ethylenediamine-Tetraacetic acid (EDTA), Tris-Hcl, Triton X-100, Glycine, Sodium Chloride
and Sodium Borohydride - Contact a licensed professional waste disposal service to dispose of this
material. Dissolve or mix the material with a combustible solvent and burn in a chemical incinerator equipped
with an afterburner and scrubber. Observe all federal, state, and local environmental regulations.
14. TRANSPORT INFORMATION
Sodium Bicarbonate, Trizma-Base, Dulbecco鈥檚 Phosphate saline, Potassium Chloride,
Ethylenediamine-Tetraacetic acid (EDTA), Tris-Hcl, Triton X-100, Glycine and Sodium Chloride -
RID/ADR: Non-hazardous for road transport. IMDG: Non-hazardous for sea transport. IATA: Non-hazardous
for air transport.
Sodium Borohydride- RID/ADR: UN#: 1426, Class: 4.3, PG: I. Proper shipping name: Sodium borohydride
IMDG: UN#: 1426, Class: 4.3, PG: I. EmS Number: 4.3-04, NFAG number: 245, Proper Shipping name:
Sodium borohydride. Marine Pollutant: No. Severe marine pollutant: No. IATA: UN#: 1426, Class: 4.3, PG: I.
Proper shipping name: Sodium borohydride. Inhalation packing group I: No
15. REGULATORY INFORMATION
Sodium Bicarbonate- Not hazardous according to directive 67/548/EC. Country specific information:
Germany: WGK: 1. Switzerland: Swiss poison class: 5.
Trizma-Base- Classification and labeling according to EU directives indication of danger: Xi. Irritant.
R-Phrases: 36/37/38, irritating to eyes, respiratory system and skin. S-Phrases: 26 36, In case of contact
with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing.
Country specific information: Germany: WGK: 2. Switzerland: Swiss poison class: FREI. Norway:
Declaration number: 67087.
Dulbecco鈥檚 Phosphate saline- Not hazardous according to directive 67/548/EC.
Potassium Chloride- Classification and labeling according to EU directives indication of danger: S-
Phrases: 22/24/25. Avoid contact with skin and eyes. Country specific information: Germany: WGK: 1.
Switzerland: Swiss poison class: 5.
Ethylenediamine-Tetraacetic acid (EDTA)- Classification and labeling according to EU directives
indication of danger: Xi. Irritant. R-Phrases: 36/38, irritating to eyes and skin. S-Phrases: 26 36, In case of
contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective
clothing. Country specific information: Germany: WGK: 2. Switzerland: Swiss poison class: 4.
Tris-Hcl- Classification and labeling according to EU directives indication of danger: Xi. Irritant. R-
Phrases: 36/37/38, irritating to eyes, respiratory system and skin. S-Phrases: 26 36, In case of contact with
eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing.
Country specific information: Germany: WGK: 1.
Triton X-100- Classification and labeling according to EU directives indication of danger: Xn. Harmful.
R-Phrases: 22 41, Harmful if swallowed. Risk of serious damage to eyes. S-Phrases: 26 36/39, In case of
contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective
Gene Bio-Application Ltd. 01/09/04
clothing and eye/face protection. Country specific information: Germany: WGK: 1. Switzerland: Swiss
poison class: 4.
Glycine- Classification and labeling according to EU directives indication of danger: S-Phrases: 22
24/25. Avoid contact with skin and eyes. Country specific information: Germany: WGK: 2. Switzerland:
Swiss poison class: FREI.
Sodium Chloride - Country specific information: Germany: WGK: 1. Switzerland: Swiss poison class: 4.
Sodium Borohydride- Classification and labeling according to EU directives indication of danger: F
T+. Highly flammable. Very toxic. R-Phrases: 15 24/25 26 34. Contact with water liberates extremely
flammable gases. Toxic in contact with skin and if swallowed. Very toxic by inhalation. Causes burns. S-
Phrases: 22 26 36/37/39 43 45. In case of contact with eyes, rinse immediately with plenty of water and seek
medical advice. Wear suitable protective clothing, gloves and eye/face protection. In case of fire, use dry
sand. Never use water. I case of accident or if you feel unwell, seek medical advice immediately (show label
where possible). Country specific information: Germany: WGK: 2. Switzerland: Swiss poison class: 3.
16. OTHER INFORMATION
Warranty: The above information is believed to be correct but does not purport to be all-inclusive and shall
be used only as a guide. The information in this document is based on the present state of our knowledge
and is applicable to the product with regard to appropriate safety precautions. It does represent any
guarantee of the properties of the product. Gene Bio-Application Ltd. shall not be held liable for any damage
resulting from handling or from contact with the above product.
Disclaimer: For R&D use only. Not for drug, household or other uses.
Gene Bio-Application Ltd. 01/09/04
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