SIGMA-ALDRICH
MATERIAL SAFETY DATA SHEET
Date Printed: 03/14/2005
Date Updated: 03/09/2004
Version 1.11
Section 1 - Product and Company Information
Product Name METHANOL, BIOTECH GRADE SOLVENT,
99.93%
Product Number 494437
Brand ALDRICH
Company Sigma-Aldrich
Street Address 3050 Spruce Street
City, State, Zip, Country SAINT LOUIS MO 63103 US
Technical Phone: 314 771 5765
Emergency Phone: 414 273 3850 Ext. 5996
Fax: 800 325 5052
Section 2 - Composition/Information on Ingredient
Substance Name CAS # SARA 313
METHANOL 67-56-1 Yes
Formula CH4O
Synonyms Alcool methylique (French) * Alcool metilico
(Italian) * Bieleski's solution * Carbinol *
Colonial Spirit * Columbian Spirit * Metanolo
(Italian) * Methanol (ACGIH) * Methyl alcohol
(DOT:OSHA) * Methylol * Methylalkohol (German) *
Methyl hydrate * Methyl hydroxide * Metylowy
alkohol (Polish) * Monohydroxymethane * Pyroxylic
Spirit * RCRA waste number U154 * Wood alcohol *
Wood naphtha * Wood Spirit * METHYL ALCOHOL *
WOOD ALCOHOL
RTECS Number: PC1400000
Section 3 - Hazards Identification
EMERGENCY OVERVIEW
Flammable (USA) Highly Flammable (EU). Toxic.
Toxic by inhalation, in contact with skin and if swallowed. Toxic:
danger of very serious irreversible effects through inhalation, in
contact with skin and if swallowed. Irritating to eyes and skin.
Target organ(s): Eyes. Kidneys.
HMIS RATING
HEALTH: 2*
FLAMMABILITY: 3
REACTIVITY: 0
NFPA RATING
HEALTH: 2
FLAMMABILITY: 3
REACTIVITY: 0
*additional chronic hazards present.
For additional information on toxicity, please refer to Section 11.
Section 4 - First Aid Measures
ORAL EXPOSURE
If swallowed, wash out mouth with water provided person is
conscious. Call a physician immediately.
INHALATION EXPOSURE
If inhaled, remove to fresh air. If not breathing give
artificial respiration. If breathing is difficult, give oxygen.
DERMAL EXPOSURE
In case of skin contact, flush with copious amounts of water for
at least 15 minutes. Remove contaminated clothing and shoes.
Call a physician.
EYE EXPOSURE
In case of contact with eyes, flush with copious amounts of
water for at least 15 minutes. Assure adequate flushing by
separating the eyelids with fingers. Call a physician.
Section 5 - Fire Fighting Measures
FLAMMABLE HAZARDS
Flammable Hazards: Yes
EXPLOSION HAZARDS
Vapor may travel considerable distance to source of ignition and
flash back. Container explosion may occur under fire conditions.
FLASH POINT
52 掳F 11 掳C Method: closed cup
EXPLOSION LIMITS
Lower: 6 % Upper: 36 %
AUTOIGNITION TEMP
385 掳C
FLAMMABILITY
N/A
EXTINGUISHING MEDIA
Suitable: Water spray. Carbon dioxide, dry chemical powder, or
appropriate foam.
FIREFIGHTING
Protective Equipment: Wear self-contained breathing apparatus
and protective clothing to prevent contact with skin and eyes.
Specific Hazard(s): Flammable liquid. Emits toxic fumes under
fire conditions.
Section 6 - Accidental Release Measures
PROCEDURE TO BE FOLLOWED IN CASE OF LEAK OR SPILL
Evacuate area. Shut off all sources of ignition.
PROCEDURE(S) OF PERSONAL PRECAUTION(S)
Wear self-contained breathing apparatus, rubber boots, and heavy
rubber gloves.
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METHODS FOR CLEANING UP
Cover with dry-lime, sand, or soda ash. Place in covered
containers using non-sparking tools and transport outdoors.
Ventilate area and wash spill site after material pickup is
complete.
ENVIRONMENTAL PRECAUTION(S)
Do not allow material to enter drains or water courses.
Section 7 - Handling and Storage
HANDLING
User Exposure: Do not breathe vapor. Avoid contact with eyes,
skin, and clothing. Avoid prolonged or repeated exposure.
STORAGE
Suitable: Keep container closed. Keep away from heat, sparks,
and open flame.
Section 8 - Exposure Controls / PPE
ENGINEERING CONTROLS
Safety shower and eye bath. Use nonsparking tools. Use only in a
chemical fume hood.
PERSONAL PROTECTIVE EQUIPMENT
Respiratory: Government approved respirator.
Hand: Compatible chemical-resistant gloves.
Eye: Chemical safety goggles.
GENERAL HYGIENE MEASURES
Wash contaminated clothing before reuse. Wash thoroughly after
handling.
EXPOSURE LIMITS, RTECS
Country Source Type Value
USA ACGIH STEL 250 PPM
Remarks: Skin
USA ACGIH TWA 200 PPM
Remarks: Skin
USA MSHA Standard-air TWA 200 PPM (260 MG/M3) (SKIN)
USA OSHA. PEL 8H TWA 200 PPM (260 MG/M3)
New Zealand OEL
Remarks: check ACGIH TLV
USA NIOSH TWA 200 PPM (SK)
STEL 250 PPM (SK)
EXPOSURE LIMITS
Country Source Type Value
Poland NDS 100 MG/M3
Poland NDSCh 300 MG/M3
Poland NDSP -
Section 9 - Physical/Chemical Properties
Appearance Physical State: Liquid
Color: Colorless
Property Value At Temperature or Pressure
Molecular Weight 32.04 AMU
pH N/A
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BP/BP Range N/A
MP/MP Range -98 掳C
Freezing Point N/A
Vapor Pressure 97.68 mmHg 20 掳C
Vapor Density 0.79 g/l
Saturated Vapor Conc. N/A
SG/Density 0.791 g/cm3
Bulk Density N/A
Odor Threshold N/A
Volatile% 100 %
VOC Content 100 %
Water Content N/A
Solvent Content N/A
Evaporation Rate N/A
Viscosity N/A
Surface Tension N/A
Partition Coefficient N/A
Decomposition Temp. N/A
Flash Point 52 掳F 11 掳C Method: closed cup
Explosion Limits Lower: 6 %
Upper: 36 %
Flammability N/A
Autoignition Temp 385 掳C
Refractive Index 1.329
Optical Rotation N/A
Miscellaneous Data N/A
Solubility Solubility in Water:Miscible.
N/A = not available
Section 10 - Stability and Reactivity
STABILITY
Stable: Stable.
Materials to Avoid: Acids, Acid chlorides, Acid anhydrides,
Oxidizing agents, Alkali metals, Reducing agents.
HAZARDOUS DECOMPOSITION PRODUCTS
Hazardous Decomposition Products: Carbon monoxide, Carbon dioxide.
HAZARDOUS POLYMERIZATION
Hazardous Polymerization: Will not occur
Section 11 - Toxicological Information
ROUTE OF EXPOSURE
Skin Contact: Causes skin irritation.
Skin Absorption: Toxic if absorbed through skin.
Eye Contact: Causes eye irritation.
Inhalation: Toxic if inhaled. Material may be irritating to
mucous membranes and upper respiratory tract.
Ingestion: Toxic if swallowed.
TARGET ORGAN(S) OR SYSTEM(S)
Eyes. Kidneys. Liver. Heart. Central nervous system.
SIGNS AND SYMPTOMS OF EXPOSURE
Methyl alcohol may be fatal or cause blindness if swallowed.
Cannot be made non-poisonous. Ingestion can cause: Nausea,
headache, and vomiting. Gastrointestinal disturbances.
Dizziness. Weakness. Confusion. Drowsiness. Unconsciousness. May
cause convulsions.
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TOXICITY DATA
Oral
Man
6422 mg/kg
LDLO
Remarks: Brain and Coverings:Changes in circulation
(hemorrhage,thrombosis, etc.). Lungs, Thorax, or
Respiration:Dyspnea. Gastrointestinal:Nausea or vomiting.
Oral
Human
428 mg/kg
LDLO
Remarks: Behavioral:Headache. Lungs, Thorax, or
Respiration:Other changes.
Oral
Human
143 mg/kg
LDLO
Remarks: Sense Organs and Special Senses (Nose, Eye, Ear, and
Taste):Eye:Optic nerve neuropathy. Lungs, Thorax, or
Respiration:Dyspnea. Gastrointestinal:Nausea or vomiting.
Oral
Rat
5628 mg/kg
LD50
Inhalation
Rat
64,000 ppm
LC50
Intraperitoneal
Rat
7529 MG/KG
LD50
Intravenous
Rat
2131 MG/KG
LD50
Oral
Mouse
7300 mg/kg
LD50
Intraperitoneal
Mouse
10765 MG/KG
LD50
Subcutaneous
Mouse
9800 MG/KG
LD50
Intravenous
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Mouse
4710 MG/KG
LD50
Oral
Monkey
7000 mg/kg
LD50
Remarks: Behavioral:Muscle weakness. Behavioral:Ataxia.
Behavioral:Coma.
Oral
Rabbit
14200 mg/kg
LD50
Skin
Rabbit
15800 mg/kg
LD50
Intraperitoneal
Rabbit
1826 MG/KG
LD50
Intravenous
Rabbit
8907 MG/KG
LD50
Intraperitoneal
Guinea pig
3556 MG/KG
LD50
Intraperitoneal
Hamster
8555 MG/KG
LD50
IRRITATION DATA
Skin
Rabbit
20 mg
24H
Remarks: Moderate irritation effect
Eyes
Rabbit
40 mg
Remarks: Moderate irritation effect
Eyes
Rabbit
100 mg
24H
Remarks: Moderate irritation effect
CHRONIC EXPOSURE - TERATOGEN
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Species: Rat
Dose: 35295 MG/KG
Route of Application: Oral
Exposure Time: (1-15D PREG)
Result: Effects on Embryo or Fetus: Fetotoxicity (except death,
e.g., stunted fetus). Effects on Newborn: Biochemical and
metabolic.
Species: Rat
Dose: 20000 PPM/7H
Route of Application: Inhalation
Exposure Time: (1-22D PREG)
Result: Specific Developmental Abnormalities: Musculoskeletal
system. Specific Developmental Abnormalities: Cardiovascular
(circulatory) system. Specific Developmental Abnormalities:
Urogenital system.
Species: Rat
Dose: 20000 PPM/7H
Route of Application: Inhalation
Exposure Time: (7-15D PREG)
Result: Specific Developmental Abnormalities: Musculoskeletal
system. Specific Developmental Abnormalities: Endocrine system.
Species: Rat
Dose: 10000 PPM/7H
Route of Application: Inhalation
Exposure Time: (7-15D PREG)
Result: Effects on Embryo or Fetus: Fetotoxicity (except death,
e.g., stunted fetus).
Species: Rat
Dose: 5200 UL/KG
Route of Application: Oral
Exposure Time: (10D PREG)
Result: Effects on Embryo or Fetus: Fetotoxicity (except death,
e.g., stunted fetus). Specific Developmental Abnormalities: Eye,
ear. Specific Developmental Abnormalities: Urogenital system.
Species: Mouse
Dose: 40 GM/KG
Route of Application: Oral
Exposure Time: (6-15D PREG)
Result: Effects on Embryo or Fetus: Fetotoxicity (except death,
e.g., stunted fetus). Specific Developmental Abnormalities:
Craniofacial (including nose and tongue).
Species: Mouse
Dose: 4 GM/KG
Route of Application: Oral
Exposure Time: (7D PREG)
Result: Specific Developmental Abnormalities: Craniofacial
(including nose and tongue). Specific Developmental
Abnormalities: Musculoskeletal system.
Species: Mouse
Dose: 1500 PPM/6H
Route of Application: Inhalation
Exposure Time: (7-9D PREG)
Result: Specific Developmental Abnormalities: Central nervous
system.
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Species: Mouse
Dose: 5000 PPM/7H
Route of Application: Inhalation
Exposure Time: (6-15D PREG)
Result: Specific Developmental Abnormalities: Central nervous
system. Specific Developmental Abnormalities: Craniofacial
(including nose and tongue).
Species: Mouse
Dose: 2000 PPM/7H
Route of Application: Inhalation
Exposure Time: (6-15D PREG)
Result: Specific Developmental Abnormalities: Musculoskeletal
system.
CHRONIC EXPOSURE - MUTAGEN
Species: Human
Dose: 300 MMOL/L
Cell Type: lymphocyte
Mutation test: DNA inhibition
Species: Rat
Route: Oral
Dose: 10 UMOL/KG
Mutation test: DNA damage
Species: Mouse
Dose: 7900 MG/L (+S9)
Cell Type: lymphocyte
Mutation test: Mutation in microorganisms
Species: Mouse
Route: Oral
Dose: 1 GM/KG
Mutation test: Cytogenetic analysis
Species: Mouse
Route: Intraperitoneal
Dose: 75 MG/KG
Mutation test: Cytogenetic analysis
CHRONIC EXPOSURE - REPRODUCTIVE HAZARD
Species: Rat
Dose: 7500 MG/KG
Route of Application: Oral
Exposure Time: (17-19D PREG)
Result: Effects on Newborn: Behavioral.
Species: Rat
Dose: 35295 MG/KG
Route of Application: Oral
Exposure Time: (1-15D PREG)
Result: Effects on Fertility: Female fertility index (e.g., #
females pregnant per # sperm positive females; # females
pregnant per # females mated ). Effects on Fertility:
Pre-implantation mortality (e.g., reduction in number of
implants per female; total number of implants per corpora
lutea). Effects on Fertility: Post-implantation mortality (e.g.,
dead and/or resorbed implants per total number of implants).
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Species: Rat
Dose: 20 GM/KG
Route of Application: Oral
Exposure Time: (6-15D PREG)
Result: Effects on Fertility: Post-implantation mortality (e.g.,
dead and/or resorbed implants per total number of implants).
Effects on Fertility: Litter size (e.g.; # fetuses per litter;
measured before birth).
Species: Rat
Dose: 200 PPM/20H
Route of Application: Oral
Exposure Time: (78W MALE)
Result: Paternal Effects: Testes, epididymis, sperm duct.
Species: Mouse
Dose: 7500 PPM/7H
Route of Application: Inhalation
Exposure Time: (6-15D PREG)
Result: Effects on Fertility: Post-implantation mortality (e.g.,
dead and/or resorbed implants per total number of implants).
Effects on Embryo or Fetus: Fetal death.
Species: Mouse
Dose: 15000 PPM
Route of Application: Inhalation
Exposure Time: (7-9D PREG)
Result: Effects on Fertility: Post-implantation mortality (e.g.,
dead and/or resorbed implants per total number of implants).
Effects on Embryo or Fetus: Fetotoxicity (except death, e.g.,
stunted fetus).
Species: Mouse
Dose: 5 GM/KG
Route of Application: Intraperitoneal
Exposure Time: (5D MALE)
Result: Paternal Effects: Spermatogenesis (including genetic
material, sperm morphology,motility, and count).
Section 12 - Ecological Information
ACUTE ECOTOXICITY TESTS
Test Type: LC50 Fish
Species: Onchorhynchus mykiss (Rainbow trout)
Time: 96 h
Value: 19,000 mg/l
Test Type: LC50 Fish
Species: Cyprinus carpio
Time: 48 h
Value: 36,000 mg/l
Test Type: EC50 Daphnia
Species: Daphnia magna
Time: 48 h
Value: 24,500 mg/l
Test Type: EC100 Daphnia
Species: Daphnia magna
Time: 24 h
Value: 10,000 mg/l
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Section 13 - Disposal Considerations
APPROPRIATE METHOD OF DISPOSAL OF SUBSTANCE OR PREPARATION
Contact a licensed professional waste disposal service to dispose
of this material. Burn in a chemical incinerator equipped with an
afterburner and scrubber but exert extra care in igniting as this
material is highly flammable. Observe all federal, state, and
local environmental regulations.
Section 14 - Transport Information
DOT
Proper Shipping Name: Methanol
UN#: 1230
Class: 3
Packing Group: Packing Group II
Hazard Label: Flammable liquid
PIH: Not PIH
IATA
Proper Shipping Name: Methanol
IATA UN Number: 1230
Hazard Class: 3
Packing Group: II
Section 15 - Regulatory Information
EU DIRECTIVES CLASSIFICATION
Symbol of Danger: F T
Indication of Danger: Highly Flammable. Toxic.
R: 11 23/24/25 39/23/24/25
Risk Statements: Highly flammable. Toxic by inhalation, in
contact with skin and if swallowed. Toxic: danger of very
serious irreversible effects through inhalation, in contact with
skin and if swallowed.
S: 7 16 36/37 45
Safety Statements: Keep container tightly closed. Keep away from
sources of ignition - no smoking. Wear suitable protective
clothing and gloves. In case of accident or if you feel unwell,
seek medical advice immediately (show the label where possible).
US CLASSIFICATION AND LABEL TEXT
Indication of Danger: Flammable (USA) Highly Flammable (EU).
Toxic.
Risk Statements: Toxic by inhalation, in contact with skin and
if swallowed. Toxic: danger of very serious irreversible effects
through inhalation, in contact with skin and if swallowed.
Irritating to eyes and skin.
Safety Statements: Keep container tightly closed. Keep away from
sources of ignition - no smoking. Take precautionary measures
against static discharges. Avoid contact with skin. Wear
suitable protective clothing and gloves. In case of accident or
if you feel unwell, seek medical advice immediately (show the
label where possible).
US Statements: Target organ(s): Eyes. Kidneys.
UNITED STATES REGULATORY INFORMATION
SARA LISTED: Yes
DEMINIMIS: 1 %
NOTES: This product is subject to SARA section 313 reporting
requirements.
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TSCA INVENTORY ITEM: Yes
CANADA REGULATORY INFORMATION
WHMIS Classification: This product has been classified in
accordance with the hazard criteria of the CPR, and the MSDS
contains all the information required by the CPR.
DSL: Yes
NDSL: No
Section 16 - Other Information
DISCLAIMER
For R&D use only. Not for drug, household or other uses.
WARRANTY
The above information is believed to be correct but does not
purport to be all inclusive and shall be used only as a guide. The
information in this document is based on the present state of our
knowledge and is applicable to the product with regard to
appropriate safety precautions. It does not represent any
guarantee of the properties of the product. Sigma-Aldrich Inc.,
shall not be held liable for any damage resulting from handling or
from contact with the above product. See reverse side of invoice
or packing slip for additional terms and conditions of sale.
Copyright 2005 Sigma-Aldrich Co. License granted to make unlimited
paper copies for internal use only.
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