AMBION, INC. Acid-Phenol: Chloroform
Catalog #:9720, 9722
Material Safety Data Sheet
Section 1-Identification of Product
9720, 9722
WHMIS Health: 3 Fire: 2 Stability: 1
Classification
Contact Information
Ambion, Inc 2130 Woodward St. Austin, TX 78744-1832 Tel: +1 512 651 0200 US Toll-free Tel: 800
888 8804 E-mail: techserv@ambion.com Web address www.ambion.com
Ambion (Europe) LTD Huntingdon, Cambidgeshire UK PE29 6XY Tel:+44 (0)1480 373 020 Fax: +44 (0)1480 373
010 E-mail: eurotech@ambion.com Web address: www.ambion.com
Emergency Contact: In Europe, call 112. In USA, call 911
Section 2-Composition/Information
Hazardous Ingredients % CAS # LD50 LC50
(Specific)
Phenol ~30-40% 108-95-2 Oral rat LD50: 317 316 mg/m3
mg/Kg
Chloroform ~10-20% 67-66-3 Oral rat LD50: 908 47702 mg/m3/4H
mg/kg
Section 3-Hazard Identification
Routes of Entry
Skin Contact Harmful if absorbed through the skin. Direct skin contact may result in white, wrinkled
discoloration, followed by severe burns. Phenol solutions may be absorbed through the
skin rapidly to cause systemic poisoning and possible death.
Skin Harmful if absorbed through the skin. Direct skin contact may result in white, wrinkled
Absorption discoloration, followed by severe burns. Phenol solutions may be absorbed through the
skin rapidly to cause systemic poisoning and possible death.
Eye Contact Causes eye irritation and possible burns. May cause chemical conjunctivitis and corneal
damage.
Inhalation May be fatal if exposed to high concentrations. May cause severe respiratory tract
irritation and possible burns. Aspiration may lead to pulmonary edema. May also cause
pallor, loss of appetite, nausea, vomiting, diarrhea, weakness, darkened urine, headache,
sweating, convulsions, cyanosis, unconsciousness, fatigue, pulmonary edema and coma.
May cause systemic effects. Inhalation at high concentrations may cause CNS depression
and asphyxiation.
Acts as a relatively potent anesthetic. Irritates respiratory tract and causes central
nervous system effects, including headache, drowsiness, and dizziness. Exposure to
higher concentrations may result in unconsciousness and even death. May cause liver
injury and blood disorders. Prolonged exposure may lead to death due to irregular
heartbeat and kidney and liver disorders.
Ingestion Harmful if swallowed. May cause central nervous system depression, characterized by
excitement, followed by headache, dizziness, drowsiness, and nausea. Advanced stages
may cause collapse, unconsciousness, coma and possible death due to respiratory failure.
May cause systemic effects. Causes digestive tract irritation with possible burns. Causes
severe burning in mouth and throat, pain in the chest and vomiting. Large quantities may
cause symptoms similar to inhalation.
[Emergency
Overview]
TOXIC BY INHALATION, IN CONTACT WITH SKIN AND IF SWALLOWED.
WHMIS Symbols
N.A.
[Potential Health
Effects]
Phenol - Human poison by ingestion. An experimental poison by ingestion, subcutaneous, intravenous, parenteral,
and intraperitoneal routes. Moderately toxic by skin contact. A severe eye and skin irritant. Questionable carcinogen
with experimental carcinogenic and neoplastigenic data. Human mutation data reported. An experimental teratogen.
Other experimental reproductive effects. Absorption of phenolic solutions through the skin may be very rapid, and
can cause death within 30 minutes to several hours by exposure of as little as 64 square inches of skin. Lesser
exposures can cause damage to the kidneys, liver, pancreas, and spleen, and edema of the lungs. Ingestion can cause
corrosion of the lips, mouth, throat, esophagus, and stomach, and gangrene. Ingestion of 1.5 g has killed. Chronic
Version 1 Page 1 of 5 Printed: 10/21/2004
Created:090904 QC Form: 1761
Revision Date: NA
AMBION, INC. Acid-Phenol: Chloroform
Catalog #:9720, 9722
Material Safety Data Sheet
exposures can cause death from liver and kidney damage. Dermatitis resulting from contact with phenol or phenol-
containing products is fairly common in industry. A common air contaminant. Combustible when exposed to heat,
flame, or oxidizers. Potentially explosive reaction with aluminum chloride + nitromethane (at 110潞C/100 bar),
formaldehyde, peroxydisulfuric acid, peroxymonosulfuric acid, sodium nitrite + heat. Violent reaction with aluminum
chloride + nitrobenzene (at 120潞C), sodium nitrate + trifluoroacetic acid, butadiene. Can react with oxidizing
materials. To fight fire, use alcohol foam, CO2, dry chemical. When heated to decomposition it emits acrid smoke
and irritating fumes
Chloroform - Confirmed carcinogen with experimental carcinogenic, neoplastigenic, and tumorigenic data. A human
poison by ingestion and inhalation. An experimental poison by ingestion and intravenous routes. Moderately toxic
experimentally by intraperitoneal and subcutaneous routes. Human systemic effects by inhalation: hallucinations and
distorted perceptions, nausea, vomiting, and other unspecified gastrointestinal effects. Human mutation data reported.
Experimental teratogenic and reproductive effects. Inhalation of the concentrated vapor causes dilation of the pupils
with reduced reaction to light, as well as reduced intraocular pressure (experimental). In the initial stages there is a
feeling of warmth of the face and body, then an irritation of the mucous membranes, conjunctiva, and skin; followed
by excitation, loss of reflexes, sensation, and consciousness. Prolonged inhalation will bring on paralysis
accompanied by cardiac-respiratory failure and finally death.
Chloroform has been widely used as an anesthetic. However, due to its toxic effects, this use is being abandoned.
Concentrations of 68,000鈥?82,000 ppm in air can kill most animals in a few minutes. 14,000 ppm may cause death
after an exposure of from 30 to 60 minutes. 5000鈥?6000 ppm can be tolerated by animals for 1 hour without serious
disturbances. The maximum concentration tolerated for several hours or for prolonged exposure with slight symptoms
is 2000鈥?2500 ppm. Prolonged administration as an anesthetic may lead to such serious effects as profound toxemia
and damage to the liver, heart, and kidneys. Experimental prolonged but light anesthesia in dogs produces a typical
hepatitis. Explosive reaction with sodium + methanol or sodium methoxide + methanol. Mixtures with sodium or
potassium are impact-sensitive explosives. Reacts violently with acetone + alkali (e.g., sodium hydroxide, potassium
hydroxide, or calcium hydroxide), Al, disilane, Li, Mg, methanol + alkali, nitrogen tetroxide, perchloric acid +
phosphorus pentoxide, potassium-tert-butoxide, sodium methylate, NaK. Incompatible with dinitrogen tetraoxide,
fluorine, metals, or triisopropylphosphine. Nonflammable. When heated to decomposition it emits toxic fumes of Cl炉
Section 4-First Aid Measures
Skin Contact Get medical aid. Immediately flush skin with plenty of soap and water for at least 15 minutes
while removing contaminated clothing and shoes. Wash clothing before reuse. Destroy
contaminated shoes.
Eye Contact Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the
upper and lower eyelids. Get medical aid immediately. Do NOT allow victim to rub or keep
eyes closed.
Inhalation Remove from exposure to fresh air immediately. If not breathing, give artificial respiration. If
breathing is difficult, give oxygen. Get medical aid. Do NOT use mouth-to-mouth
resuscitation.
Ingestion Do NOT induce vomiting. If victim is conscious and alert, give 2-4 cupfuls of milk or water.
Never give anything by mouth to an unconscious person. Get medical aid immediately.
Note to Physician: Because kidney and liver effects may be delayed, keep victim under observation for 24 to
48 hr. Administration of fluids may help to prevent kidney failure. Obtain blood glucose,
Urinalysis, liver function tests, chest x-ray, and monitor cardiac function and fluidelectrolyte
status. Monitor liver and kidney function for 4 to 5 days after exposure.
Disulfiram, its metabolites, and a high carbohydrate diet appear to protect somewhat against
chloroform toxicity. Do not give adrenalin! Tests may show increased bilirubin, ketosis,
lowered blood prothombin, and fibrogen.
Section 5-Fire Fighting Measures
Flammable Flammable
Means of Extinction CO2, H2O, Foam, Dry Chemical
Flashpoint (oC) and Method Not Tested. Phenol-79C (174F)
Upper Flammable Limit (% by volume) Not Tested. Phenol-8.6%
Lower Flammable Limit (% by volume) Not Tested. Phenol-1.3%
Autoignition Temperature (oC) Not Tested. Phenol-715C (1319F)
Explosion Data 鈥? Sensitivity to Impact Not Tested
Explosion Data 鈥? Sensitivity to Static Not Tested
Discharge
Hazardous Combustion Products Not Tested
NFPA Health: 3 Fire: 2 Stability: 1
Section 6-Accidental Release Measures
Leak and Spill
Procedures
Version 1 Page 2 of 5 Printed: 10/21/2004
Created:090904 QC Form: 1761
Revision Date: NA
AMBION, INC. Acid-Phenol: Chloroform
Catalog #:9720, 9722
Material Safety Data Sheet
Ventilate area of leak or spill. Wear appropriate personal protective equipment as specified in Section 8. Isolate
hazard area. Keep unnecessary and unprotected personnel from entering. Contain and recover liquid when possible.
Collect liquid in an appropriate container or absorb with an inert material (e. g., vermiculite, dry sand, earth), and
place in a chemical waste container. Do not use combustible materials, such as saw dust. Do not flush to sewer! US
Regulations (CERCLA) require reporting spills and releases to soil, water and air in excess of reportable quantities.
The toll free number for the US Coast Guard National Response Center is (800) 424-8802.
Section 7-Handling and Storage
Handling Procedures Wash hands, face, forearms and neck when exiting restricted areas. Shower, dispose of outer
and Equipment clothing, change to clean garments at the end of the day.
Avoid cross-contamination of street clothes. Wash hands before eating and do not eat, drink,
or smoke in workplace.
Storage Requirements Keep in a tightly closed light-resistant container, stored in a cool, dry, ventilated area.
Protect against physical damage. Isolate from incompatible substances. Wear special
protective equipment (Sec. 8) for maintenance break-in or where exposures may exceed
established exposure levels.
Section 8-Exposure Control/Personal Protection
Exposure Limits
ACGIH TLV Phenol - 5 ppm TWA; skin - potential for cutaneous absorption
Chloroform - 10 ppm TWA
OSHA PEL Phenol - 5 ppm TWA; 19 mg/m3 TWA
Chloroform - 50 ppm Ceiling; 240 mg/m3 Ceiling
Other (specify) N.A.
Engineering Controls (specific)
General Facilities storing or utilizing this material should be equipped with an eyewash facility and a
safety shower. Use adequate ventilation to keep airborne concentrations low.
Local A system of local and/or general exhaust is recommended to keep employee exposures as
Exhaust low as possible. Local exhaust ventilation is generally preferred because it can control the
emissions of the contaminant at its source, preventing dispersion of it into the general work
area. Please refer to the ACGIH document, Industrial Ventilation, A Manual of
Recommended Practices, most recent edition, for details.
Other N.A.
Personal Protective Equipment (specific)
Gloves Neoprene gloves
Respirator A respiratory protection program that meets OSHA's 29 CFR 1910.134 and ANSI Z88.2
requirements or European Standard EN 149 must be followed whenever workplace
conditions warrant a respirator's use.
Eye Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's
eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166.
Maintain eye wash fountain and quick-drench facilities in work area.
Footwear Closed toe shoes
Clothing Wear appropriate protective clothing to prevent skin exposure.
Other Chemical fume hood, face shield
Section 9-Physical and Chemical Properties
Physical State Liquid
Odor and Appearance Strong / White- off white
Odor Threshold (ppm) Not Tested
Specific Gravity Not Tested
Vapor Density (Air=1) Not Tested
Vapor Pressure (mmHg) Not Tested
Evaporation Rate Not Tested
Boiling Point (oC) Not Tested
Freezing Point (oC) Not Tested
pH 4.5
Coefficient of Water/Oil Not Tested
Distribution
[Solubility in Water] Not Tested
Section 10-Stability and Reactivity
Chemical Stability Stable
Version 1 Page 3 of 5 Printed: 10/21/2004
Created:090904 QC Form: 1761
Revision Date: NA
AMBION, INC. Acid-Phenol: Chloroform
Catalog #:9720, 9722
Material Safety Data Sheet
Incompatible with other Aluminum chloride, nitrobenzene, peroxomonosulfuric
substances acid, peroxydisulfuric acid, calcium hypochlorite, sodium nitrite, acetaldehyde, 1,3-
butadiene, boron trifluoride diethyl ether, strong oxidizing agents, isocyanates,
nitrides (e.g. potassium nitride, sodium nitride), acids, sodium + methanol, sodium
methoxide + methanol, sodium, potassium, acetone + alkali, aluminum, disilane,
lithium, magnesium, potassium-tert -butoxide, sodium methylate, dinitrogen
tetraoxide, fluorine, metals, triisopropylphosphine, reducing agents, hydrogen
trisulfide, acid anhydrides, acid chlorides.
Reactivity Not Tested
Hazardous Decomposition Hydrogen chloride, phosgene, carbon monoxide, irritating and toxic fumes and
Products gases, carbon dioxide, chlorine.
Section 11-Toxicological Information
Acute Effects Harmful if inhaled, swallowed, or absorbed through skin.
Chronic Effects May cause cancer
Irritancy of Product Inhalation may result in spasm, inflammation and edema of the larynx and
bronchi, chemical Phenumonitis and Pulmonary Edema.
Skin Sensitization Not Tested
Respiratory Sensitization Not Tested
Carcinogenicity
IARC (1,2A, or 2B) CAS# 108-95-2: IARC Group 3 - not classifiable CAS# 67-66-3:
ACGIH (A1, A2, or A3) A4 - Not Classifiable as a Human Carcinogen
A3 - Animal Carcinogen
Reproductive Toxicity CAS# 108-95-2: Oral, rat: TDLo = 300 mg/kg (female 6-15 day(s) after conception)
Fertility - post-implantation mortality (e.g. dead and/or resorbed implants per total
number of implants)
Teratogenicity CAS# 108 -95-2: Oral, rat: TDLo = 1200 mg/kg (female 6-15 day(s) after conception)
Effects on Embryo or Fetus - fetotoxicity (except death, e.g., stunted fetus).; Oral, mouse:
TDLo = 4 gm/kg (female 6-15 day(s) after conception) Specific Developmental
Abnormalities - musculoskeletal system.
Embryotoxicity No Information
Mutagenicity CAS# 108-95-2: Mutation Test Systems - not otherwise specified: Human, HeLa cell =
17 mg/L.; DNA Inhibition: Human, HeLa cell = 1 mmol/L.; Mutation Test Systems 鈥? not
otherwise specified: Human, Lymphocyte = 5 umol/L.; Sister Chromatid Exchange:
Human,
Name of Synergistic N.A.
Products/Effects
Section 12-Ecological Information
Aquatic Water flea Daphnia: EC50=12 mg/l; 48 -hour; CAS# 108-95-2: Unspecified flea
Toxicity Daphnia: EC50=4.0 mg/l; 96-hour; CAS# 108-95-2: Unspecified Fathead Minnow: LC50 >
50 mg/l; 1 Hr; CAS# 108-95-2 Static @ 18-22掳C Fathead Minnow: TLm = 41 mg/L; 48-
hour; CAS# 108-95-2: Flow-through @ 15掳C Bluegill/Sunfish: TLm = 19 / 5.7 mg/L; 96 Hr;
CAS# 108-95-2:
Flow-through If released to the environment, phenol's primary removal mechanism is
biodegradation which is generally rapid (days). If phenol is released to soil, it will readily
leach and biodegrade. The biodegradation in soil is generally rapid with half-lives of under 5
days even in subsurface soils.
Section 13-Disposal Considerations
Waste Chemical waste generators must determine whether a discarded chemical is classified as a
Disposal hazardous waste. US EPA guidelines for the classification determination are listed in 40
CFR Parts 261.3. Additionally, waste generators must consult state and local hazardous
waste regulations to ensure complete and accurate classification.
Section 14-Transport Information
Special Shipping Information
PIN Toxic Liquid, Organic N.O.S.
TDG Packing Group III
[DOT] Hazard Class 6.1 UN 2810
[IMO] Not listed
[ICAO] Not listed
Section 15-Regulatory Information
[WHMIS
Classification]
Version 1 Page 4 of 5 Printed: 10/21/2004
Created:090904 QC Form: 1761
Revision Date: NA
AMBION, INC. Acid-Phenol: Chloroform
Catalog #:9720, 9722
Material Safety Data Sheet
[OSHA] Phenol - CAS# 108-95-2: Effective Date: 6/1/87; Sunset Date: 6/1/97
Chloroform - CAS# 67-66-3: Effective Date: 6/1/87; Sunset Date: 6/1/97
[SARA] Phenol - CAS# 108-95-2: 500 lb TPQ (lower threshold); 10,000 lb TPQ (upper
threshold); 1000 lb EPCRA RQ
Phenol - 108-95-2: acute, chronic, flammable.
Phenol - This material contains Phenol (CAS# 108-95-2, 52 0%),which is subject to the
reporting requirements of Section 313 of SARA Title III and 40 CFR Part 373.
Chloroform - CAS# 67-66-3: 10,000 lb TPQ; 10 lb EPCRA RQ
Chloroform - CAS # 67-66-3: acute, chronic.
Chloroform - This material contains Chloroform (CAS# 67-66-3, 46 0%), which is
subject to the reporting requirements of Section 313 of SARA Title III and 40 CFR Part
373.
[TSCA] Phenol - CAS# 108-95-2 is listed on the TSCA inventory.
Chloroform - 67-66-3 is listed on the TSCA inventory.
Section 16-Other Information
This bulletin is for your guidance and is based upon information and tests believed to be reliable. Ambion makes no
guarantee of the accuracy or completeness of the data and shall not be liable for any damages thereto. The data are
offered solely for your consideration, investigation, and verification. These suggestions should not be confused with
state, municipal, or insurance requirements, or with national safety codes and constitute no warranty. Any use of
these data and information must be determined by the user to be in accordance with applicable federal, state, and local
regulations.
Version 1 Page 5 of 5 Printed: 10/21/2004
Created:090904 QC Form: 1761
Revision Date: NA
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