AMBION, INC. 10X TBE Buffer
Catalog #:9863, 9865
Material Safety Data Sheet
Section 1-Identification of Product
Catalog Number: 9863, 9865 For research Health: 2 Fire: 0 Stability: 1
use only.
Synonyms:
Contact Information
Ambion, Inc., 2130 Woodward St. Austin, TX 78744-1832 Tel: +1 512 651 0200 US Toll-free Tel: 800 888 8804 E-
mail: techserv@ambion.com Web address www.ambion.com
Ambion (Europe) LTD Huntingdon, Cambidgeshire UK PE29 6XY Tel:+44 (0)1480 373 020 Fax: +44 (0)1480 373
010 E-mail: eurotech@ambion.com Web address: www.ambion.com
Emergency Contact: In Europe, call 112. In USA, call 911
Section 2-Composition/Information
Hazardous Ingredients (Specific) % CAS # R-Statement S-Statement
Tris 1-20 77-86-1 R: 36/37/38 S: 36/37/39
Boric Acid 1-20 10043-35-3 R: 36/37/38 S: 36/37/39
Section 3-Hazard Identification
Routes of Entry
Skin Contact Causes irritation to skin. Symptoms include redness, itching, and
pain.
Skin Absorption Boric Acid: Not significantly absorbed through the intact skin.
Readily absorbed through damaged or burned skin. Symptoms of skin
absorption parallel inhalation and ingestion.
Eye Contact Causes irritation, redness, and pain.
Inhalation Tris: Causes irritation to the respiratory tract. Symptoms may include
coughing, shortness of breath.
Boric Acid: Causes irritation to the mucous membranes of the
respiratory tract. May be absorbed from the mucous membranes, and
depending on the amount of exposure could result in the development
of nausea, vomiting, diarrhea, drowsiness, rash, headache, fall in
body temperature, low blood pressure, renal injury, cyanosis, coma,
and death.
Ingestion Tris: Causes irritation and reddening to the mucous membranes of the
mouth, esophagus, and gastrointestinal tract. Symptoms may include
nausea, vomiting and diarrhea. Large oral doses may cause weakness,
collapse, blood clotting and coma. Estimated lethal dose: 50 grams.
Boric Acid: Symptoms parallel absorption via inhalation. Adult fatal
dose reported at 5 to >30 grams.
[Emergency Overview]
WARNING! HARMFUL IF SWALLOWED OR INHALED. CAUSES IRRITATION TO SKIN, EYES AND
RESPIRATORY TRACT. AFFECTS CENTRAL NERVOUS SYSTEM, LIVER AND KIDNEYS.
WHMIS Symbols
N.A.
[Potential Health Effects]
Tris:
Moderately toxic by ingestion and intravenous routes. When heated to decomposition it emits toxic fumes on Nox.
Boric Acid:
A human poison by ingestion and possibly other routes. Moderately toxic by skin contact and subcutaneous routes in
humans. Poison experimentally by inhalation and subcutaneous routes. Moderately toxic experimentally by
intraperitoneal and intravenous routes. Human systemic effects: anorexia, changes in kidney tubules, nausea or
vomiting, wakefulness. Ingestion or absorption by other routes may also cause diarrhea, abdominal cramps,
erythematous lesions on skin and mucous membranes, circulatory collapse, tachycardia, cyanosis, delirium,
convulsions, and coma. Death has occurred from ingestion of less than 5 g in infants, and from 5 to 20 g in adults.
Chronic exposure may result in borism (dry skin, eruptions, and gastrointestinal disturbances). Experimental
reproductive effects. Mutation data reported. A human skin irritant.
Section 4-First Aid Measures
Skin Contact Get medical aid. Flush skin with plenty of water for at least 15 minutes while removing
contaminated clothing and shoes.
EHS-MSDS-10X TBE Buffer-9863, 9865-01 Page 1 of 4 051106
AMBION, INC. 10X TBE Buffer
Catalog #:9863, 9865
Material Safety Data Sheet
Eye Contact Flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower
eyelids. Get medical aid immediately.
Inhalation Get medical aid immediately. Remove from exposure and move to fresh air immediately. If not
breathing, give artificial respiration. If breathing is difficult, give oxygen.
Ingestion Never give anything by mouth to an unconscious person. Do NOT induce vomiting. If conscious
and alert, rinse mouth and drink 2-4 cupfuls of milk or water. Get medical aid if irritation or
symptoms occur.
Section 5-Fire Fighting Measures
Flammable Not considered to be a fire hazard.
Means of Extinction Water spray, dry chemical, alcohol foam, or carbon
dioxide.
Flashpoint (oC) and Method Not Tested
Upper Flammable Limit (% by volume) Not Tested
Lower Flammable Limit (% by volume) Not Tested
Autoignition Temperature (oC) Not Tested
Explosion Data 鈥? Sensitivity to Impact Not Tested
Explosion Data 鈥? Sensitivity to Static Discharge Not Tested
Hazardous Combustion Products Burning may produce carbon monoxide, carbon dioxide,
nitrogen oxides.
Boric Acid: Loses chemically combined water upon
heating, forming metaboric acid (HBO2) at 212-221F, then
pyroboric acid (H2B4O7) at 285-320F, and Boric
anhydride at higher temperatures.
NFPA Health: 2 Fire: 0 Stability: 1
Section 6-Accidental Release Measures
Leak and Spill Procedures
Ventilate area of leak or spill. Remove all sources of ignition. Wear appropriate personal protective equipment as
specified in Section 8. Isolate hazard area. Keep unnecessary and unprotected personnel from entering. Contain and
recover liquid when possible. Collect liquid in an appropriate container or absorb with an inert material (e. g.,
vermiculite, dry sand, earth), and place in a chemical waste container. Do not use combustible materials, such as
sawdust. Do not flush to sewer!
Section 7-Handling and Storage
Handling Procedures and Use with adequate ventilation. Avoid contact with eyes, skin, and clothing. Keep
Equipment container tightly closed. Avoid ingestion and inhalation.
Storage Requirements Keep in a tightly closed container, stored in a cool, dry, ventilated area. Protect
against physical damage. Isolate from incompatible substances.
Section 8-Exposure Control/Personal Protection
Exposure Limits
ACGIH TLV Not Listed
OSHA PEL Not Listed
Other (specify) N.A.
Engineering Controls (specific)
General Facilities storing or utilizing this material should be equipped with an eyewash
facility and a safety shower. Use adequate ventilation to keep airborne
concentrations low.
Local Exhaust A system of local and/or general exhaust is recommended to keep employee
exposures as low as possible. Local exhaust ventilation is generally preferred
because it can control the emissions of the contaminant at its source, preventing
dispersion of it into the general work area. Please refer to the ACGIH document,
Industrial Ventilation, A Manual of Recommended Practices, most recent
edition, for details.
Other N.A.
Personal Protective Equipment (specific)
Gloves Wear impervious gloves.
Respirator A respiratory protection program that meets OSHA's 29 CFR 1910.134 and
ANSI Z88.2 requirements or European Standard EN 149 must be followed
whenever workplace conditions warrant a respirator's use.
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AMBION, INC. 10X TBE Buffer
Catalog #:9863, 9865
Material Safety Data Sheet
Eye Wear appropriate protective eyeglasses or chemical safety goggles as described
by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European
Standard EN166. Maintain eye wash fountain and quick-drench facilities in work
area.
Footwear Wear closed toe shoes.
Clothing Wear appropriate protective clothing to prevent skin exposure.
Other N.A
Section 9-Physical and Chemical Properties
Physical State Liquid
Odor and Appearance Semi-Clear
Odor Threshold (ppm) Not Tested
Specific Gravity Not Tested
Vapor Density (Air=1) Not Tested
Vapor Pressure (mmHg) Not Tested
Evaporation Rate Not Tested
Boiling Point (oC) Not Tested
Freezing Point (oC) Not Tested
pH Not Tested
Coefficient of Water/Oil Distribution Not Tested
[Solubility in Water] Not Tested
Section 10-Stability and Reactivity
Chemical Stability Stable under ordinary conditions of use and storage.
Incompatible with other substances Copper, brass, aluminum, and oxidizing agents.
Potassium, acetic anhydride, alkalis, carbonates, and hydroxides.
Reactivity Not Tested
Hazardous Decomposition Products Burning may produce carbon monoxide, carbon dioxide, nitrogen
oxides.
Boric Acid: Loses chemically combined water upon heating,
forming metaboric acid (HBO2) at 212-221F, then pyroboric acid
(H2B4O7) at 285-320F, and Boric anhydride at higher
temperatures.
Section 11-Toxicological Information
Acute Effects May be harmful by inhalation, ingestion, or skin absorption. Causes eye and
skin irritation. Material is irritating to mucous membranes and upper
respiratory tract.
Chronic Effects Prolonged absorption causes weight loss, vomiting, diarrhea, skin rash,
convulsions and anemia. Liver and particularly the kidneys may be susceptible.
Studies of dogs and rats have shown that infertility and damage to testes can
result from acute or chronic ingestion of boric acid. Evidence of toxic effects
on the human reproductive system is inadequate.
Irritancy of Product Skin, eyes, respiratory tract, mucous membranes of the mouth, esophagus and
gastrointestinal tract.
Skin Sensitization Not Tested
Respiratory Sensitization Not Tested
Tris LD50 Oral rat: 5900 mg/kg
Tris LC50 Not Tested
Boric Acid LD50 Oral rat: 2660 mg/kg
Boric Acid LC50 Not Tested
Carcinogenicity
IARC (1,2A, or 2B) Not Listed
ACGIH (A1, A2, or A3) Not Listed
Reproductive Toxicity Boric Acid: Investigated as a reproductive effector. Experimental
reproductive effects.
Teratogenicity Not Listed
Embryotoxicity Not Listed
Mutagenicity Boric Acid: Investigated as a mutagen, tumorigen. Mutation data
reported.
Name of Synergistic Not Listed
Products/Effects
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AMBION, INC. 10X TBE Buffer
Catalog #:9863, 9865
Material Safety Data Sheet
Section 12-Ecological Information
Aquatic Toxicity Boric Acid:
The EC50/48-hour values for daphnia are over 100mg/l. This material may be
toxic to aquatic life.
Section 13-Disposal Considerations
Waste Disposal Chemical waste generators must determine whether a discarded chemical is
classified as a hazardous waste. US EPA guidelines for the classification
determination are listed in 40 CFR Parts 261.3. Additionally, waste generators
must consult state and local hazardous waste regulations to ensure complete and
accurate classification.
Section 14-Transport Information
Special Shipping Information
PIN Not Listed
TDG Not Listed
[DOT] Not Listed
[IMO] Not Listed
[ICAO] Not Listed
Section 15-Regulatory Information
[WHMIS Classification] Not Listed
[OSHA] Not Listed
[SARA] Tris:
SARA 302- RQ: No, TPQ: No
SARA 313- List: No, Chemical Catg.: No
SARA 311/312- Acute: Yes, Chronic: No, Fire: No, Pressure: No,
Reactivity: No
Boric Acid:
SARA 302- RQ: No, TPQ: No
SARA 313- List: No, Chemical Catg.: No
SARA 311/312- Acute: Yes, Chronic: Yes, Fire: No, Pressure: No,
Reactivity: No
[TSCA] Tris: Reported in the EPA TSCA Inventory.
Boric Acid: Reported in EPA TSCA Inventory.
R: 36/37/38 Irritating to eyes, respiratory system and skin.
S: 36/37/39 Wear suitable protective clothing, gloves and eye/face protection.
Section 16-Other Information
This bulletin is for your guidance and is based upon information and tests believed to be reliable. Ambion makes no
guarantee of the accuracy or completeness of the data and shall not be liable for any damages thereto. The data are
offered solely for your consideration, investigation, and verification. These suggestions should not be confused with
state, municipal, or insurance requirements, or with national safety codes and constitute no warranty. Any use of
these data and information must be determined by the user to be in accordance with applicable federal, state, and local
regulations.
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