AMBION, INC. RNase-Free Buffers
1M Tris pH 8.0
Material Safety Data Sheet
Section 1-Identification of Product
Catalog Number: 9855G, 9856 For research Health: 1 Fire: 1 Stability: 0
use only.
Synonyms:
Contact Information
Ambion, Inc 2130 Woodward St. Austin, TX 78744-1832 Tel: +1 512 651 0200 US Toll-free Tel: 800 888 8804 E-
mail: techserv@ambion.com Web address www.ambion.com
Ambion (Europe) LTD Huntingdon, Cambidgeshire UK PE29 6XY Tel:+44 (0)1480 373 020 Fax: +44 (0)1480 373
010 E-mail: eurotech@ambion.com Web address: www.ambion.com
Emergency Contact: In Europe, call 112. In USA, call 911
Section 2-Composition/Information
Hazardous Ingredients (Specific) % CAS # R-Statement S-Statement
Tris 12 77-86-1 R:36/37/38 S:36/37/39
Section 3-Hazard Identification
Routes of Entry
Skin Contact Causes irritation to skin. Symptoms include redness, itching, and
pain.
Skin Absorption Causes skin irritation.
Eye Contact Causes irritation, redness, and pain.
Inhalation Causes irritation to the respiratory tract. Symptoms may include
coughing, shortness of breath.
Ingestion Mild alkali. Causes irritation and reddening to the mucous
membranes of the mouth, esophagus, and gastrointestinal tract.
Symptoms may include nausea, vomiting and diarrhea. Large oral
doses may cause weakness, collapse, blood clotting and coma.
Estimated lethal dose: 50 grams.
[Emergency Overview]
WARNING! HARMFUL IF SWALLOWED OR INHALED. CAUSES IRRITATION TO SKIN, EYES AND
RESPIRATORY TRACT.
WHMIS Symbols
N.A.
[Potential Health Effects]
N.A.
Section 4-First Aid Measures
Skin Contact Immediately flush skin with plenty of soap and water for at least 15 minutes. Remove
contaminated clothing and shoes. Get medical attention. Wash clothing before reuse.
Thoroughly clean shoes before reuse.
Eye Contact Immediately flush eyes with plenty of water for at least 15 minutes, lifting upper and lower
eyelids occasionally. Get medical attention.
Inhalation Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give
oxygen. Get medical attention.
Ingestion Induce vomiting immediately as directed by medical personnel. Never give anything by mouth
to an unconscious person. Get medical attention.
Section 5-Fire Fighting Measures
Flammable Slightly flammable
Means of Extinction Water spray, dry chemical, alcohol foam, or carbon
dioxide.
Flashpoint (oC) and Method Not Tested
Upper Flammable Limit (% by volume) Not Tested
Lower Flammable Limit (% by volume) Not Tested
Autoignition Temperature (oC) Not Tested
Explosion Data 鈥? Sensitivity to Impact Not Tested
Explosion Data 鈥? Sensitivity to Static Discharge Not Tested
Hazardous Combustion Products Not Tested
NFPA Health: 1 Fire: 1 Stability: 0
Section 6-Accidental Release Measures
Leak and Spill Procedures
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AMBION, INC. RNase-Free Buffers
1M Tris pH 8.0
Material Safety Data Sheet
Ventilate area of leak or spill. Remove all sources of ignition. Wear appropriate personal protective equipment as
specified in Section 8. Isolate hazard area. Keep unnecessary and unprotected personnel from entering. Contain and
recover liquid when possible. Collect liquid in an appropriate container or absorb with an inert material (e. g.,
vermiculite, dry sand, earth), and place in a chemical waste container. Do not use combustible materials, such as
sawdust. Do not flush to sewer!
Section 7-Handling and Storage
Handling Procedures and Use with adequate ventilation. Avoid contact with eyes, skin, and clothing. Keep
Equipment container tightly closed. Avoid ingestion and inhalation.
Storage Requirements Keep in a tightly closed container, stored in a cool, dry, ventilated area. Protect
against physical damage. Isolate from any source of heat or ignition. Isolate from
oxidizing materials.
Section 8-Exposure Control/Personal Protection
Exposure Limits
ACGIH TLV Not Listed
OSHA PEL Not Listed
Other (specify) N.A.
Engineering Controls (specific)
General Facilities storing or utilizing this material should be equipped with an eyewash
facility and a safety shower. Use adequate ventilation to keep airborne
concentrations low.
Local Exhaust A system of local and/or general exhaust is recommended to keep employee
exposures as low as possible. Local exhaust ventilation is generally preferred
because it can control the emissions of the contaminant at its source, preventing
dispersion of it into the general work area.
Other N.A.
Personal Protective Equipment (specific)
Gloves Wear impervious gloves.
Respirator A respiratory protection program that meets OSHA's 29 CFR 1910.134 and
ANSI Z88.2 requirements or European Standard EN 149 must be followed
whenever workplace conditions warrant a respirator's use.
Eye Wear appropriate protective eyeglasses or chemical safety goggles as described
by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European
Standard EN166. Maintain eye wash fountain and quick-drench facilities in work
area.
Footwear Wear closed toe shoes.
Clothing Wear appropriate protective clothing to prevent skin exposure.
Other N.A.
Section 9-Physical and Chemical Properties
Physical State Liquid
Odor and Appearance Clear
Odor Threshold (ppm) Not Tested
Specific Gravity Not Tested
Vapor Density (Air=1) Not Tested
Vapor Pressure (mmHg) Not Tested
Evaporation Rate Not Tested
Boiling Point (oC) Not Tested
Freezing Point (oC) Not Tested
pH 8.0
Coefficient of Water/Oil Distribution Not Tested
[Solubility in Water] Not Tested
Section 10-Stability and Reactivity
Chemical Stability Stable under ordinary conditions of use and storage.
Incompatible with other substances Copper, brass, aluminum, and oxidizing agents
Reactivity Not Tested
Hazardous Decomposition Products Thermal decomposition may produce carbon monoxide, carbon
dioxide, nitrogen oxides.
Section 11-Toxicological Information
Acute Effects Not Tested
Chronic Effects Not Tested
Irritancy of Product Irritating to eyes, skin, respiratory, and gastrointestinal system.
EHS-MSDS-1MTrispH8-9855-01 Page 2 of 3 01/10/06
AMBION, INC. RNase-Free Buffers
1M Tris pH 8.0
Material Safety Data Sheet
Skin Sensitization Not Tested
Respiratory Sensitization Not Tested
LD50 Oral rat: 5900 mg/kg.
LC50 Not Tested
Carcinogenicity
IARC (1,2A, or 2B) Not Listed
ACGIH (A1, A2, or A3) Not Listed
Reproductive Toxicity Not Listed
Teratogenicity Not Listed
Embryotoxicity Not Listed
Mutagenicity Not Listed
Name of Synergistic Not Listed
Products/Effects
Section 12-Ecological Information
Aquatic Toxicity Not Tested
Section 13-Disposal Considerations
Waste Disposal Chemical waste generators must determine whether a discarded chemical is
classified as a hazardous waste. US EPA guidelines for the classification
determination are listed in 40 CFR Parts 261.3. Additionally, waste generators
must consult state and local hazardous waste regulations to ensure complete and
accurate classification.
Section 14-Transport Information
Special Shipping Information
PIN Not Listed
TDG Not Listed
[DOT] Not Listed
[IMO] Not Listed
[ICAO] Not Listed
Section 15-Regulatory Information
[WHMIS Classification] Not Listed
[OSHA] Not Listed
[SARA] Not Listed
[TSCA] Not Listed
R:36/37/38 Irritating to eyes, respiratory system and skin.
S:36/37/39 Wear suitable protective clothing, gloves and eye/face protection.
Section 16-Other Information
This bulletin is for your guidance and is based upon information and tests believed to be reliable. Ambion makes no
guarantee of the accuracy or completeness of the data and shall not be liable for any damages thereto. The data are
offered solely for your consideration, investigation, and verification. These suggestions should not be confused with
state, municipal, or insurance requirements, or with national safety codes and constitute no warranty. Any use of
these data and information must be determined by the user to be in accordance with applicable federal, state, and local
regulations.
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