850 Lincoln Centre Drive
Foster City, CA 94404
U.S.A.
T 650.570.6667
F 650.572.2743
www.appliedbiosystems.com
Dear Customer:
Ambion is supplying this letter to you as required by Section 313 of Title III of the Superfund Amendments and
Reauthorization Act commonly called SARA 313. Sec. 372.45 of SARA 313 requires that if we sell or otherwise distribute a
mixture or trade name product containing a toxic chemical or chemicals subject to the reporting requirements of SARA 313,
we must provide a written notification with the first shipment of each calendar year.
You will continue to receive this notification annually when the product is re-shipped, or if there is a change to the MSDS
(Material Safety Data Sheet). MSDSs are an important part of product information and are provided as required by OSHA
(Occupational Safety and Health Act), under the OSHA Hazard Communication Standard (29 CFR 1910.1200), for
hazardous chemicals. MSDSs include health, handling and hazard information which should be communicated to your
employees, customers and others who handle this product. Additional MSDS information can be found online at
http://www.ambion.com/techlib/detail_results.html
Additional Requirements:
鈥? If you repackage or otherwise redistribute this product, a notice with this SARA 313 information should be also sent
to the recipient of this product.
鈥? This notification must remain attached to the accompanying MSDS. Any copying or redistribution of the MSDS
shall include a copy of this notification.
This product contains:
Chemical Name CAS # W/W%
鈮? 60
PHENOL 108-95-2
Please review the attached Material Safety Data Sheet for risks and handling precautions believed to be associated with this
material. If you have any additional questions or require additional information, please feel free to contact us. Thank you for
your continued support of Ambion and Applied Biosystems products.
Best regards,
Global Chemical Compliance and Risk Management
850 Lincoln Centre Drive
Foster City, CA 94404
1-800-327-3002 x2230
Chemical_Compliance@appliedbiosystems.com
MSDS PART NUMBER: 4378512 US
OHS PART NUMBER: 00232036
Revision number : B
Page 1 of 12
MATERIAL SAFETY DATA SHEET
1. CHEMICAL PRODUCT AND COMPANY IDENTIFICATION
APPLIED BIOSYSTEMS APPLIED BIOSYSTEMS
850 LINCOLN CENTRE DRIVE 7 KINGSLAND GRANGE, WOOLSTON
FOSTER CITY, CA 94404 WARRINGTON, CHESHIRE WA1 4SR
(650) 570-6667 (USA) +44 1925 82 56 50 (UK)
EMERGENCY CONTACT: EMERGENCY CONTACT:
1-800-424-9300 (NORTH AMERICA) +1-703-527-3887, REVERSE CHARGES
(INTERNATIONAL)
SUBSTANCE: TRI REAGENT庐
TRADE NAMES/SYNONYMS:
US MSDS P/N 4378512; P/N 9738; P/N AM9738; 00232036
PRODUCT USE: For Research Use Only. Not for use in diagnostic procedures.
CREATION DATE: Jul 25 2006
REVISION DATE: Oct 24 2006
2. COMPOSITION, INFORMATION ON INGREDIENTS
COMPONENT: PHENOL
CAS NUMBER: 108-95-2
PERCENTAGE: 30-60
COMPONENT: TRADE SECRET 00232117
CAS NUMBER: Not assigned.
PERCENTAGE: 30-60
COMPONENT: TRADE SECRET 00232118
CAS NUMBER: Not assigned.
PERCENTAGE: 10-30
COMPONENT: TRADE SECRET 00232119
MSDS PART NUMBER: 4378512 US
OHS PART NUMBER: 00232036
Revision number : B
Page 2 of 12
CAS NUMBER: Not assigned.
PERCENTAGE: <10
COMPONENT: TRADE SECRET
CAS NUMBER: Not assigned.
PERCENTAGE: <10
3. HAZARDS IDENTIFICATION
NFPA RATINGS (SCALE 0-4): HEALTH=3 FIRE=1 REACTIVITY=0
EMERGENCY OVERVIEW:
COLOR: purple
PHYSICAL FORM: solution
ODOR: sweet odor, medicinal odor, tar odor
MAJOR HEALTH HAZARDS: harmful on contact with the skin, respiratory tract
burns, skin burns, eye burns, mucous membrane burns, central nervous system
depression
PHYSICAL HAZARDS: Contact with acids or bleach liberates toxic gases.
POTENTIAL HEALTH EFFECTS:
INHALATION:
SHORT TERM EXPOSURE: burns, cough, changes in blood pressure, ringing in the
ears, nausea, vomiting, diarrhea, stomach pain, difficulty breathing,
irregular heartbeat, headache, drowsiness, dizziness, disorientation,
hyperactivity, hallucinations, tremors, loss of coordination, bluish skin
color, lung congestion, blood disorders, convulsions, coma, death
LONG TERM EXPOSURE: burns, rash, vomiting, diarrhea, loss of appetite,
headache, dizziness, disorientation, hormonal disorders, kidney damage,
liver damage
SKIN CONTACT:
SHORT TERM EXPOSURE: burns, absorption may occur, ringing in the ears,
nausea, vomiting, diarrhea, stomach pain, irregular heartbeat, headache,
lung congestion, blood disorders, kidney damage, liver damage, nerve damage,
convulsions, coma
LONG TERM EXPOSURE: burns, skin disorders, rash, itching, nausea, vomiting,
diarrhea, stomach pain, loss of appetite, headache, dizziness,
disorientation, hormonal disorders, kidney damage, liver damage
MSDS PART NUMBER: 4378512 US
OHS PART NUMBER: 00232036
Revision number : B
Page 3 of 12
EYE CONTACT:
SHORT TERM EXPOSURE: burns, blurred vision, eye damage, blindness
LONG TERM EXPOSURE: burns, blurred vision, eye damage, blindness
INGESTION:
SHORT TERM EXPOSURE: burns, changes in blood pressure, nausea, vomiting,
diarrhea, stomach pain, difficulty breathing, irregular heartbeat, headache,
drowsiness, dizziness, disorientation, hyperactivity, emotional
disturbances, tremors, loss of coordination, dilated pupils or pin-point
pupils, bluish skin color, blood disorders, nerve damage, convulsions, coma,
death
LONG TERM EXPOSURE: burns, skin disorders, rash, nausea, vomiting, diarrhea,
loss of appetite, headache, dizziness, disorientation, hyperactivity,
hormonal disorders, internal bleeding, kidney damage, liver damage,
reproductive effects
4. FIRST AID MEASURES
INHALATION: If adverse effects occur, remove to uncontaminated area. Give
artificial respiration if not breathing. If breathing is difficult, oxygen
should be administered by qualified personnel. Get immediate medical
attention.
SKIN CONTACT: Wash skin with soap and water for at least 15 minutes while
removing contaminated clothing and shoes. Get immediate medical attention.
Discard contaminated clothing and footwear. Destroy contaminated shoes.
EYE CONTACT: Immediately flush eyes with plenty of water for at least 15
minutes. Then get immediate medical attention.
INGESTION: If swallowed, drink plenty of water, do NOT induce vomiting. Get
immediate medical attention.
NOTE TO PHYSICIAN: For inhalation, consider oxygen. Avoid gastric lavage or
emesis.
MSDS PART NUMBER: 4378512 US
OHS PART NUMBER: 00232036
Revision number : B
Page 4 of 12
5. FIRE FIGHTING MEASURES
FIRE AND EXPLOSION HAZARDS: Slight fire hazard.
EXTINGUISHING MEDIA: carbon dioxide, regular dry chemical, regular foam, water
FIRE FIGHTING: Move container from fire area if it can be done without risk.
Cool containers with water spray until well after the fire is out. Do not
get water directly on material. Avoid inhalation of material or combustion
by-products. Stay upwind and keep out of low areas.
FLASH POINT: 230 F (110 C)
FLAMMABILITY CLASS (OSHA): IIIB
6. ACCIDENTAL RELEASE MEASURES
OCCUPATIONAL RELEASE:
Do not touch spilled material. Stop leak if possible without personal risk.
Small spills: Absorb with sand or other non-combustible material. Small dry
spills: Collect spilled material in appropriate container for disposal. Keep
unnecessary people away, isolate hazard area and deny entry. Notify Local
Emergency Planning Committee and State Emergency Response Commission for
release greater than or equal to RQ (U.S. SARA Section 304). If release occurs
in the U.S. and is reportable under CERCLA Section 103, notify the National
Response Center at (800)424-8802 (USA) or (202)426-2675 (USA).
7. HANDLING AND STORAGE
STORAGE: Store and handle in accordance with all current regulations and
standards. Store in a cool, dry place. Store in a well-ventilated area. Avoid
direct sunlight. Notify State Emergency Response Commission for storage or use
at amounts greater than or equal to the TPQ (U.S. EPA SARA Section 302). SARA
Section 303 requires facilities storing a material with a TPQ to participate
in local emergency response planning (U.S. EPA 40 CFR 355.30). See original
container for storage recommendations. Keep separated from incompatible
substances.
MSDS PART NUMBER: 4378512 US
OHS PART NUMBER: 00232036
Revision number : B
Page 5 of 12
8. EXPOSURE CONTROLS, PERSONAL PROTECTION
EXPOSURE LIMITS:
PHENOL:
5 ppm (19 mg/m3) OSHA TWA (skin)
5 ppm ACGIH TWA (skin)
5 ppm (19 mg/m3) NIOSH recommended TWA 10 hour(s) (skin)
15.6 ppm (60 mg/m3) NIOSH recommended ceiling 15 minute(s) (skin)
VENTILATION: Provide local exhaust or process enclosure ventilation system.
Ensure compliance with applicable exposure limits.
EYE PROTECTION: Wear splash resistant safety goggles with a faceshield.
Provide an emergency eye wash fountain and quick drench shower in the
immediate work area.
CLOTHING: Wear appropriate chemical resistant clothing.
GLOVES: Wear appropriate chemical resistant gloves.
PROTECTIVE MATERIAL TYPES: rubber, neoprene
RESPIRATOR: Under conditions of frequent use or heavy exposure, respiratory
protection may be needed. Respiratory protection is ranked in order from
minimum to maximum. Consider warning properties before use.
Any dust, mist, and fume respirator.
Any air-purifying respirator with a high-efficiency particulate filter.
Any powered, air-purifying respirator with a dust, mist, and fume filter.
Any powered, air-purifying respirator with a high-efficiency particulate
filter.
For Unknown Concentrations or Immediately Dangerous to Life or Health -
Any supplied-air respirator with full facepiece and operated in a
pressure-demand or other positive-pressure mode in combination with a
separate escape supply.
Any self-contained breathing apparatus with a full facepiece.
MSDS PART NUMBER: 4378512 US
OHS PART NUMBER: 00232036
Revision number : B
Page 6 of 12
9. PHYSICAL AND CHEMICAL PROPERTIES
PHYSICAL STATE: liquid
COLOR: purple
PHYSICAL FORM: solution
ODOR: sweet odor, medicinal odor, tar odor
BOILING POINT: Not available
FREEZING POINT: Not available
VAPOR PRESSURE: >0.35 mmHg @ 25 C
VAPOR DENSITY: Not available
SPECIFIC GRAVITY: Not available
WATER SOLUBILITY: soluble
PH: Not available
VOLATILITY: Not available
ODOR THRESHOLD: Not available
EVAPORATION RATE: Not available
COEFFICIENT OF WATER/OIL DISTRIBUTION: Not available
SOLVENT SOLUBILITY:
Soluble: glycerol, alkali hydroxide solutions, dimethyl sulfoxide
10. STABILITY AND REACTIVITY
REACTIVITY: Stable at normal temperatures and pressure.
CONDITIONS TO AVOID: Avoid heat, flames, sparks and other sources of ignition.
May ignite or explode on contact with combustible materials.
INCOMPATIBILITIES: acids, aldehydes, alkalis, chlorates, metals, metal salts,
nitrates, nitrite salts, oxidizing materials, peroxides
HAZARDOUS DECOMPOSITION:
Thermal decomposition products: oxides of carbon, oxides of nitrogen, oxides
of sulfur, oxides of sodium, cyanide compounds
POLYMERIZATION: Will not polymerize.
MSDS PART NUMBER: 4378512 US
OHS PART NUMBER: 00232036
Revision number : B
Page 7 of 12
11. TOXICOLOGICAL INFORMATION
TRI REAGENT庐:
TOXICITY DATA:
673 mg/kg oral-rat LD50
ACUTE TOXICITY LEVEL:
Moderately Toxic: ingestion
PHENOL:
IRRITATION DATA:
500 mg/24 hour(s) skin-rabbit severe; 535 mg open skin-rabbit severe; 100 mg
skin-rabbit mild; 5 mg eyes-rabbit severe; 5 mg/30 second(s) rinsed
eyes-rabbit mild
TOXICITY DATA:
316 mg/m3 inhalation-rat LC50; 630 mg/kg skin-rabbit LD50; 317 mg/kg
oral-rat LD50
CARCINOGEN STATUS: IARC: Human Inadequate Evidence, Animal Inadequate
Evidence, Group 3; ACGIH: A4 -Not Classifiable as a Human Carcinogen
LOCAL EFFECTS:
Corrosive: inhalation, skin, eye, ingestion
ACUTE TOXICITY LEVEL:
Highly Toxic: inhalation
Toxic: dermal absorption, ingestion
TARGET ORGANS: central nervous system
MEDICAL CONDITIONS AGGRAVATED BY EXPOSURE: convulsive disorders, kidney
disorders, liver disorders, respiratory disorders, skin disorders and
allergies
TRADE SECRET 00232117:
LOCAL EFFECTS:
Irritant: skin
Corrosive: eye
ACUTE TOXICITY LEVEL: Insufficient Data.
MEDICAL CONDITIONS AGGRAVATED BY EXPOSURE: kidney disorders, respiratory
disorders
MSDS PART NUMBER: 4378512 US
OHS PART NUMBER: 00232036
Revision number : B
Page 8 of 12
TRADE SECRET 00232118:
TOXICITY DATA:
750 mg/kg oral-rat LD50
LOCAL EFFECTS:
Irritant: inhalation, skin, eye
ACUTE TOXICITY LEVEL:
Moderately Toxic: ingestion
TRADE SECRET 00232119:
IRRITATION DATA:
500 mg/24 hour(s) skin-rabbit mild; 10 mg eyes-rabbit mild
TOXICITY DATA:
>30 gm/m3/1 hour(s) inhalation-rat LC50; >10 gm/kg skin-rabbit LD50; 3530
mg/kg oral-rat LD50
ACUTE TOXICITY LEVEL:
Moderately Toxic: ingestion
Additional toxicological data is available on the component(s) of this product. Please call 650-638-5635
or contact MSDS_Inquiry_CCRM@appliedbiosystems.com for more information.
12. ECOLOGICAL INFORMATION
Not available
13. DISPOSAL CONSIDERATIONS
Dispose in accordance with all applicable regulations.
14. TRANSPORT INFORMATION
U.S. DOT 49 CFR 172.101:
PROPER SHIPPING NAME: Corrosive liquids, n.o.s. (GUANIDINE
THIOCYANATE/PHENOL SOLUTION)
ID NUMBER: UN1760
HAZARD CLASS OR DIVISION: 8
MSDS PART NUMBER: 4378512 US
OHS PART NUMBER: 00232036
Revision number : B
Page 9 of 12
PACKING GROUP: II
LABELING REQUIREMENTS: 8
CANADIAN TRANSPORTATION OF DANGEROUS GOODS:
SHIPPING NAME: Corrosive liquid, n.o.s. (GUANIDINE THIOCYANATE/PHENOL
SOLUTION)
UN NUMBER: UN1760
CLASS: 8
PACKING GROUP/RISK GROUP: II
LAND TRANSPORT ADR:
PROPER SHIPPING NAME: Corrosive liquid, n.o.s. (GUANIDINE THIOCYANATE/PHENOL
SOLUTION)
UN NUMBER: UN1760
CLASS: 8
CLASSIFICATION CODE: C9
PACKING GROUP: II
LABELS: 8
LAND TRANSPORT RID:
PROPER SHIPPING NAME: Corrosive liquid, n.o.s. (GUANIDINE THIOCYANATE/PHENOL
SOLUTION)
UN NUMBER: UN1760
CLASS: 8
CLASSIFICATION CODE: C9
PACKING GROUP: II
LABELS: 8
AIR TRANSPORT IATA:
PROPER SHIPPING NAME: Corrosive liquid, n.o.s. (GUANIDINE THIOCYANATE/PHENOL
SOLUTION)
UN/ID NUMBER: UN1760
CLASS OR DIVISION: 8
HAZARD LABELS: 8
PACKING GROUP: II
AIR TRANSPORT ICAO:
PROPER SHIPPING NAME: Corrosive liquid, n.o.s. (GUANIDINE THIOCYANATE/PHENOL
SOLUTION)
MSDS PART NUMBER: 4378512 US
OHS PART NUMBER: 00232036
Revision number : B
Page 10 of 12
UN NUMBER: UN1760
CLASS OR DIVISION: 8
LABELS: 8
UN PACKING GROUP: II
MARITIME TRANSPORT IMDG:
PROPER SHIPPING NAME: Corrosive liquid, n.o.s. (GUANIDINE THIOCYANATE/PHENOL
SOLUTION)
UN NUMBER: UN1760
CLASS OR DIVISION: 8
PACKING GROUP: II
15. REGULATORY INFORMATION
U.S. REGULATIONS:
SARA TITLE III SECTION 302 EXTREMELY HAZARDOUS SUBSTANCES (40 CFR 355.30):
PHENOL: 500/10000 LBS TPQ
SARA TITLE III SECTION 304 EXTREMELY HAZARDOUS SUBSTANCES (40 CFR 355.40):
PHENOL: 1000 LBS RQ
SARA TITLE III SARA SECTIONS 311/312 HAZARDOUS CATEGORIES (40 CFR 370.21):
ACUTE: Yes
CHRONIC: No
FIRE: No
REACTIVE: No
SUDDEN RELEASE: No
SARA TITLE III SECTION 313 (40 CFR 372.65):
PHENOL
OSHA PROCESS SAFETY (29CFR1910.119): Not regulated.
STATE REGULATIONS:
California Proposition 65: Not regulated.
MSDS PART NUMBER: 4378512 US
OHS PART NUMBER: 00232036
Revision number : B
Page 11 of 12
CANADIAN REGULATIONS:
WHMIS CLASSIFICATION: This product has been classified in accordance with
the hazard criteria of the CPR and the MSDS contains all of the
information required by the CPR.
NATIONAL INVENTORY STATUS:
U.S. INVENTORY (TSCA): Not listed on inventory. For purposes of 40 CFR
720.36, this product is for Research and Development (R&D) Use Only.
TSCA 12(b) EXPORT NOTIFICATION: Not listed.
16. OTHER INFORMATION
Copyright 1984-2006 MDL Information Systems, Inc. All rights reserved.
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